BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26571

Title: 1H, 13C and 15N NMR assignments of a calcium-binding protein(EhCaBP6) from Entamoeba histolytica   PubMed: 26377206

Deposition date: 2015-05-06 Original release date: 2015-09-28

Authors: Verma, Deepshikha; Chary, Kandala

Citation: Verma, Deepshikha; Bhattacharya, Alok; Chary, Kandala. "1H, 13C and 15N NMR assignments of a calcium-binding protein from Entamoeba histolytica"  Biomol. NMR Assignments 10, 67-70 (2016).

Assembly members:
EhCaBP6, polymer, 156 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Entamoeba histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21C

Entity Sequences (FASTA):
EhCaBP6: MSMEIEAPNANTQKIRDCFN FYDRDYDGKIDVKQLGTLIR SLGCAPTEDEVNSYIKEFAI EGETFQIEQFELIMEREQSK PDTREIKLRKAFEVFDQDKD GKIKASDLAHNLTTVGDKMT KEEVEKVFSILGITMESDID LATFLKLVALHHHHHH

Data sets:
Data typeCount
13C chemical shifts306
15N chemical shifts144
1H chemical shifts613

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EhCaBP61
2CALCIUM ION2

Entities:

Entity 1, EhCaBP6 156 residues - Formula weight is not available

1   METSERMETGLUILEGLUALAPROASNALA
2   ASNTHRGLNLYSILEARGASPCYSPHEASN
3   PHETYRASPARGASPTYRASPGLYLYSILE
4   ASPVALLYSGLNLEUGLYTHRLEUILEARG
5   SERLEUGLYCYSALAPROTHRGLUASPGLU
6   VALASNSERTYRILELYSGLUPHEALAILE
7   GLUGLYGLUTHRPHEGLNILEGLUGLNPHE
8   GLULEUILEMETGLUARGGLUGLNSERLYS
9   PROASPTHRARGGLUILELYSLEUARGLYS
10   ALAPHEGLUVALPHEASPGLNASPLYSASP
11   GLYLYSILELYSALASERASPLEUALAHIS
12   ASNLEUTHRTHRVALGLYASPLYSMETTHR
13   LYSGLUGLUVALGLULYSVALPHESERILE
14   LEUGLYILETHRMETGLUSERASPILEASP
15   LEUALATHRPHELEULYSLEUVALALALEU
16   HISHISHISHISHISHIS

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EhCaBP6, [U-100% 13C], 1 mM; Tris.HCl 50 mM; NaCl 50 mM; CaCl2 4 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, BRUKER - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts