BMRB Entry 26568

Name: The structure of the SOLE element of oskar mRNA
Published: 2015-05-04

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PDB ID: 5a17
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26568
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The structure of the SOLE element of oskar mRNA

Deposition date:

2015-04-28

Original release date:

2015-05-04

Authors:

Simon, Bernd; Masiewicz, P.; Ephrussi, A.; Carlomagno, T.

Citation:

Citation: Simon, Bernd; Masiewicz, P.; Ephrussi, A.; Carlomagno, T.. "The structure of the SOLE element of oskar mRNA" RNA 21, 1444-1453 (2015).
PubMed: 26089324

Assembly members:

Assembly members:
OSKAR_MRNA, polymer, 32 residues, 10376.25762 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OSKAR_MRNA: GACGAUAUCGAGCAUCAAGA GUGAAUAUCGUC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	213
15N chemical shifts	38
1H chemical shifts	251
31P chemical shifts	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OSKAR MRNA	1

Entities:

Entity 1, OSKAR MRNA 32 residues - 10376.25762 Da.

1

G

A

C

G

A

U

A

U

C

G

2

A

G

C

A

U

C

A

G

A

3

G

U

G

A

U

A

U

C

G

4

U

C

Samples:

sample_1: OSKAR MRNA, [U-13C; U-15N], 0.35 ± 0.05 mM

sample_conditions_1: ionic strength: 0.0 mM; pH: 6.400; pressure: 1 atm; temperature: 308.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
HBCNB/HSCNB	sample_1	solution	sample_conditions_1
HCCH-COSY-TOCSY	sample_1	solution	sample_conditions_1
3D EDITED NOESY	sample_1	solution	sample_conditions_1
HBCNB/HSCNB	sample_1	solution	sample_conditions_1
HCCH-COSY-TOCSY	sample_1	solution	sample_conditions_1
3D 13C EDITED NOESY	sample_1	solution	sample_conditions_1
2D NOESY	sample_1	solution	sample_conditions_1
2D HNN-COSY	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, PDBe - chemical shift assignment

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

FELIX vany, Accelrys Software Inc. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	P25158
PDB	5A17 5A18