BMRB Entry 26565

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Notch1 Transmembrane Domain Segment.
Published: 2016-01-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26565

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Notch1 Transmembrane Domain Segment.

Deposition date:

2015-04-21

Original release date:

2016-01-06

Authors:

Deatherage, Catherine; Lu, Zhenwei; Kim, Ji-Hun; Sanders, Charles

Citation:

Citation: Deatherage, Catherine; Lu, Zhenwei; Kim, Ji-Hun; Sanders, Charles. "Notch Transmembrane Domain: Secondary Structure and Topology" Biochemistry 54, 3565-3568 (2015).
PubMed: 26023825

Assembly members:

Assembly members:
Notch1, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTrcHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Notch1: MGHHHHHHVQSETVEPPPPA QLHFMYVAAAAFVLLFFVGC GVLLSRKRRRQHGQLWFPE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	137
15N chemical shifts	46
1H chemical shifts	46

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Notch1 transmembrane segement	1

Entities:

Entity 1, Notch1 transmembrane segement 59 residues - Formula weight is not available

Residues 1-8 represent a non-native his tag. This is the transmembrane segment of the human Notch1 receptor. As such, residue V9 is analogous to V1721 in the full length Notch 1 Protein.

1

MET

GLY

HIS

VAL

GLN

2

SER

GLU

THR

VAL

GLU

PRO

ALA

3

GLN

LEU

HIS

PHE

MET

TYR

VAL

ALA

4

ALA

PHE

VAL

LEU

PHE

VAL

GLY

CYS

5

GLY

VAL

LEU

SER

ARG

LYS

ARG

6

GLN

HIS

GLY

GLN

LEU

TRP

PHE

PRO

GLU

Samples:

sample_1: Notch1 Transmembrane segment, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 20 mM; imidazole 500 mM; DHPC 65 mM; DMPC 195 mM; DTT 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 900 MHz

UNP	P46531
AlphaFold	Q5SXM3