BMRB Entry 26564

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26564

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Title:
Y-family polymerase Dbh (dinB homolog) from Sulfolobus acidocaldarius 1H, 15N, and 13C chemical shifts
Deposition date:
2015-04-20
Original release date:
2015-07-14
Authors:
Moro, Sean; Cocco, Melanie
Citation:

Citation: Moro, Sean; Cocco, Melanie. "1H, 13C, and 15N backbone resonance assignments of the full-length 40 kDa S. acidocaldarius Y-family DNA polymerase, dinB homolog"  Biomol. NMR Assignments 9, 441-445 (2015).
PubMed: 26154586

Assembly members:

Assembly members:
Dbh_C31S, polymer, 360 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sulfolobus acidocaldarius   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus acidocaldarius

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKKT7-H

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dbh_C31S: MHHHHHHIVIFVDFDYFFAQ VEEVLNPQYKGKPLVVSVYS GRTKTSGAVATANYEARKLG VKAGMPIIKAMQIAPSAIYV PMRKPIYEAFSNRIMNLLNK HADKIEVASIDEAYLDVTNK VEGNFENGIELARKIKQEIL EKEKITVTVGVAPNKILAKI IADKSKPNGLGVIRPTEVQD FLNELDIDEIPGIGSVLARR LNELGIQKLRDILSKNYNEL EKITGKAKALYLLKLAQNKY SEPVENKSKIPHGRYLTLPY NTRDVKVILPYLKKAINEAY NKVNGIPMRITVIAIMEDLD ILSKGKKFKHGISIDNAYKV AEDLLRELLVRDKRRNVRRI GVKLDNIIINKTNLSDFFDI

Data typeCount
13C chemical shifts1189
15N chemical shifts568
1H chemical shifts631

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DbhS C31S1

Entities:

Entity 1, DbhS C31S 360 residues - Formula weight is not available

Residue 31 is Cys in WT Dbh Mutated to Ser.

1   METHISHISHISHISHISHISILEVALILE
2   PHEVALASPPHEASPTYRPHEPHEALAGLN
3   VALGLUGLUVALLEUASNPROGLNTYRLYS
4   GLYLYSPROLEUVALVALSERVALTYRSER
5   GLYARGTHRLYSTHRSERGLYALAVALALA
6   THRALAASNTYRGLUALAARGLYSLEUGLY
7   VALLYSALAGLYMETPROILEILELYSALA
8   METGLNILEALAPROSERALAILETYRVAL
9   PROMETARGLYSPROILETYRGLUALAPHE
10   SERASNARGILEMETASNLEULEUASNLYS
11   HISALAASPLYSILEGLUVALALASERILE
12   ASPGLUALATYRLEUASPVALTHRASNLYS
13   VALGLUGLYASNPHEGLUASNGLYILEGLU
14   LEUALAARGLYSILELYSGLNGLUILELEU
15   GLULYSGLULYSILETHRVALTHRVALGLY
16   VALALAPROASNLYSILELEUALALYSILE
17   ILEALAASPLYSSERLYSPROASNGLYLEU
18   GLYVALILEARGPROTHRGLUVALGLNASP
19   PHELEUASNGLULEUASPILEASPGLUILE
20   PROGLYILEGLYSERVALLEUALAARGARG
21   LEUASNGLULEUGLYILEGLNLYSLEUARG
22   ASPILELEUSERLYSASNTYRASNGLULEU
23   GLULYSILETHRGLYLYSALALYSALALEU
24   TYRLEULEULYSLEUALAGLNASNLYSTYR
25   SERGLUPROVALGLUASNLYSSERLYSILE
26   PROHISGLYARGTYRLEUTHRLEUPROTYR
27   ASNTHRARGASPVALLYSVALILELEUPRO
28   TYRLEULYSLYSALAILEASNGLUALATYR
29   ASNLYSVALASNGLYILEPROMETARGILE
30   THRVALILEALAILEMETGLUASPLEUASP
31   ILELEUSERLYSGLYLYSLYSPHELYSHIS
32   GLYILESERILEASPASNALATYRLYSVAL
33   ALAGLUASPLEULEUARGGLULEULEUVAL
34   ARGASPLYSARGARGASNVALARGARGILE
35   GLYVALLYSLEUASPASNILEILEILEASN
36   LYSTHRASNLEUSERASPPHEPHEASPILE

Samples:

15N_DbhS: Dbh C31S, [U-15N], 0.5 mM; D2O, [U-100% 2H], 10%

13C-15N-2H_DbhS: Dbh C31S, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Arg-_15N_DbhS: Dbh C31S, [U-15N; NA-Arg], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Lys_15N_DbhS: Dbh C31S, [U-15N; NA-Lys], 0.5 mM; D2O, [U-100% 2H], 10%

14N_His_-_15N_DbhS: Dbh C31S, [U-15N; NA-His], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Gly_15N_DbhS: Dbh C31S, [U-15N; NA-Gly], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Asn_15N_DbhS: Dbh C31S, [U-15N; NA-Asn], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Met_-_15N_DbhS: Dbh C31S, [U-15N; NA-Met], 0.5 mM; D2O, [U-100% 2H], 10%

15N_Asp_-_14N_DbhS: Dbh C31S, [U-15N-Asp], 0.5 mM; D2O, [U-100% 2H], 10%

14N_Arg_Asn_Gly_Ser_-_15N_DbhS: Dbh C31S, [U-15N; NA-Arg,Asn,Gly,Ser], 0.5 mM; D2O, [U-100% 2H], 10%

13CLeu_15N-2H_DbhS: Dbh C31S, [U-100% 13C-Leu; U-100% 15N; U-80% 2H], 0.5 mM; D2O, [U-100% 2H], 10%

35C: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 308 K

50C: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 323 K

45C: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 318 K

55C: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 328 K

65C: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 338 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_DbhSisotropic35C
2D 1H-15N HSQC15N_DbhSisotropic45C
2D 1H-15N HSQC15N_DbhSisotropic50C
2D 1H-15N HSQC15N_DbhSisotropic55C
2D 1H-15N HSQC15N_DbhSisotropic65C
3D 1H-15N NOESY15N_DbhSisotropic50C
3D HNCO13C-15N-2H_DbhSisotropic50C
3D HNCACB13C-15N-2H_DbhSisotropic50C
3D HN(CO)CA13C-15N-2H_DbhSisotropic50C
3D HN(CA)CO13C-15N-2H_DbhSisotropic50C
3D HNCA13C-15N-2H_DbhSisotropic35C
3D HN(CO)CA13C-15N-2H_DbhSisotropic35C
2D 1H-15N HSQC14N_Lys_15N_DbhSisotropic45C
2D 1H-15N HSQC14N_Lys_15N_DbhSisotropic55C
2D 1H-15N HSQC14N_Arg-_15N_DbhSisotropic45C
2D 1H-15N HSQC14N_Arg-_15N_DbhSisotropic55C
2D 1H-15N HSQC14N_His_-_15N_DbhSisotropic35C
2D 1H-15N HSQC14N_His_-_15N_DbhSisotropic55C
2D 1H-15N HSQC14N_His_-_15N_DbhSisotropic45C
2D 1H-15N HSQC15N_Asp_-_14N_DbhSisotropic50C
2D 1H-15N HSQC14N_Met_-_15N_DbhSisotropic50C
2D 1H-15N HSQC14N_Arg_Asn_Gly_Ser_-_15N_DbhSisotropic50C
2D 1H-15N HSQC14N_Gly_15N_DbhSisotropic50C
2D 1H-15N HSQC13C-15N-2H_DbhSisotropic35C
3D HNCO13CLeu_15N-2H_DbhSisotropic50C

Software:

NMRPipe v7.8, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPNMR_Analysis v2.3.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks