BMRB Entry 26562

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26562

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Title:
NMR assignments of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus
Deposition date:
2015-04-16
Original release date:
2016-01-06
Authors:
Mueller, Geoffrey; London, Robert
Citation:

Citation: Mueller, Geoffrey; London, Robert. "Serological, genomic, and structural analyses of the major mite allergen Der p 23"  Clin. Exp. Allergy 46, 365-376 (2015).
PubMed: 26602749

Assembly members:

Assembly members:
Der_p_23, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: European house dust mite   Taxonomy ID: 6956   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Dermatophagoides pteronyssinus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDest566

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Der_p_23: SFTKFECPSRFGYFADPKDP HKFYICSNWEAVHKDCPGNT RWNEDEETCT

Data sets:
Data typeCount
13C chemical shifts139
15N chemical shifts41
1H chemical shifts207

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1major mite allergen Der p 231

Entities:

Entity 1, major mite allergen Der p 23 50 residues - Formula weight is not available

1   SERPHETHRLYSPHEGLUCYSPROSERARG
2   PHEGLYTYRPHEALAASPPROLYSASPPRO
3   HISLYSPHETYRILECYSSERASNTRPGLU
4   ALAVALHISLYSASPCYSPROGLYASNTHR
5   ARGTRPASNGLUASPGLUGLUTHRCYSTHR

Samples:

sample_1: Der p 23, [U-100% 13C; U-100% 15N], 0.5 ± 0.2 mM; sodium chloride 68.5 mM; potassium chloride 1.35 mM; sodium phosphate 5 mM; potassium phosphate 0.9 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.10185 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
hbcbcgcdhdsample_1isotropicsample_conditions_1
hbcbcgcdcehesample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

GB ACB46292

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks