BMRB Entry 26554

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26554

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Title:
Chemical shift assignments of K2 dehydrin in the presence of SDS micelles
Deposition date:
2015-04-11
Original release date:
2017-08-24
Authors:
Graether, Steffen; Warnica, Josephine; Atkinson, John
Citation:

Citation: Clarke, Matthew; Boddington, Kelly; Warnica, Josephine; Atkinson, John; McKenna, Sarah; Madge, Jeffrey; Barker, Christine; Graether, Steffen. "Structural and Functional Insights into the Cryoprotection of Membranes by the Intrinsically Disordered Dehydrins"  J. Biol. Chem. 290, 26900-26913 (2015).
PubMed: 26370084

Assembly members:

Assembly members:
K2, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: riverbank grape   Taxonomy ID: 96939   Superkingdom: Eukaryota   Kingdom: Viridipplantae   Genus/species: Vitis riparia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K2: MKEKIKERIPGMGRKDEQKQ TSATSTPGQGQQQKGMMEKI KEKLPGAH

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts44
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K21

Entities:

Entity 1, K2 48 residues - Formula weight is not available

1   METLYSGLULYSILELYSGLUARGILEPRO
2   GLYMETGLYARGLYSASPGLUGLNLYSGLN
3   THRSERALATHRSERTHRPROGLYGLNGLY
4   GLNGLNGLNLYSGLYMETMETGLULYSILE
5   LYSGLULYSLEUPROGLYALAHIS

Samples:

sample_1: K2, [U-100% 13C; U-100% 15N], 0.2 – 1.0 mM; NaCl 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.010 M; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPNMR, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks