BMRB Entry 26552

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Plasmodium falciparum Immune Mapped Protein 1 (PfIMP1)
Published: 2016-01-06

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26552

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Plasmodium falciparum Immune Mapped Protein 1 (PfIMP1)

Deposition date:

2015-04-08

Original release date:

2016-01-06

Authors:

Williams, Felix; Kerry, Louise

Citation:

Citation: Benjamin, Stefi; Williams, Felix; Kerry, Louise; Matthews, Stephen. "NMR assignment of the immune mapped protein 1 (IMP1) homologue from Plasmodium falciparum" Biomol. NMR Assignments 9, 393-395 (2015).
PubMed: 25947350

Assembly members:

Assembly members:
PfIMP1, polymer, 181 residues, Formula weight is not available

Natural source:

Natural source: Common Name: apicomplexans Taxonomy ID: 36329 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-NTH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PfIMP1: MHHHHHHSSGVDLGTENLYF QSMMSEEKGAYLVFDNASNG TLFIVWKKEKVENALMFIKP TKEVPEFKFVNRNGKNELIR NLQSDKKLFYSGICQFVKEA KDIKGKLTLLQHFDSSFPIK VDLYFLKGSKVMPLNTGEPF VVQDIDAMSVLPKGSSSLKV KTMAKDMFVSRGNTEGASIS F

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	693
15N chemical shifts	145
1H chemical shifts	1087

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PfIMP1 monomer	1

Entities:

Entity 1, PfIMP1 monomer 181 residues - Formula weight is not available

1

MET

HIS

SER

GLY

2

VAL

ASP

LEU

GLY

THR

GLU

ASN

LEU

TYR

PHE

3

GLN

SER

MET

SER

GLU

LYS

GLY

ALA

4

TYR

LEU

VAL

PHE

ASP

ASN

ALA

SER

ASN

GLY

5

THR

LEU

PHE

ILE

VAL

TRP

LYS

GLU

LYS

6

VAL

GLU

ASN

ALA

LEU

MET

PHE

ILE

LYS

PRO

7

THR

LYS

GLU

VAL

PRO

GLU

PHE

LYS

PHE

VAL

8

ASN

ARG

ASN

GLY

LYS

ASN

GLU

LEU

ILE

ARG

9

ASN

LEU

GLN

SER

ASP

LYS

LEU

PHE

TYR

10

SER

GLY

ILE

CYS

GLN

PHE

VAL

LYS

GLU

ALA

11

LYS

ASP

ILE

LYS

GLY

LYS

LEU

THR

LEU

12

GLN

HIS

PHE

ASP

SER

PHE

PRO

ILE

LYS

13

VAL

ASP

LEU

TYR

PHE

LEU

LYS

GLY

SER

LYS

14

VAL

MET

PRO

LEU

ASN

THR

GLY

GLU

PRO

PHE

15

VAL

GLN

ASP

ILE

ASP

ALA

MET

SER

VAL

16

LEU

PRO

LYS

GLY

SER

LEU

LYS

VAL

17

LYS

THR

MET

ALA

LYS

ASP

MET

PHE

VAL

SER

18

ARG

GLY

ASN

THR

GLU

GLY

ALA

SER

ILE

SER

19

PHE

Samples:

sample_1: PfIMP1, [U-100% 13C; U-100% 15N], 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz