BMRB Entry 26545

Title:
Prostate associated gene 4 (PAGE4) phosphorylated
Deposition date:
2015-03-20
Original release date:
2016-10-05
Authors:
He, Yanan; Chen, Yihong; Mooney, Steven; Rajagopalan, Krithika; Bhargava, Ajay; Sacho, Elizabeth; Weninger, Keith; Kulkarni, Prakash; Orban, John
Citation:

Citation: He, Yanan; Chen, Yihong; Mooney, Steven; Rajagopalan, Krithika; Bhargava, Ajay; Sacho, Elizabeth; Weninger, Keith; Bryan, Philip; Kulkarni, Prakash; Orban, John. "Phosphorylation-induced Conformational Ensemble Switching in an Intrinsically Disordered Cancer/Testis Antigen"  J. Biol. Chem. 290, 25090-25102 (2015).
PubMed: 26242913

Assembly members:

Assembly members:
Prostate_associated_gene_4_phosphorylated, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: bacterial over-expression   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts353
15N chemical shifts88
1H chemical shifts399

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prostate associated gene 4 phosphorylated1

Entities:

Entity 1, Prostate associated gene 4 phosphorylated 102 residues - Formula weight is not available

1   METSERALAARGVALARGSERARGSERARG
2   GLYARGGLYASPGLYGLNGLUALAPROASP
3   VALVALALAPHEVALALAPROGLYGLUSER
4   GLNGLNGLUGLUPROPROTHRASPASNGLN
5   ASPILEGLUPROGLYGLNGLUARGGLUGLY
6   TPOPROPROILEGLUGLUARGLYSVALGLU
7   GLYASPCYSGLNGLUMETASPLEUGLULYS
8   THRARGSERGLUARGGLYASPGLYSERASP
9   VALLYSGLULYSTHRPROPROASNPROLYS
10   HISALALYSTHRLYSGLUALAGLYASPGLY
11   GLNPRO

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 200 mM; potassium phosphate 100 mM; D2O 10%; H2O 90%; NaN3 10 mM; DTT 0.1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNCANNHsample_1isotropicsample_conditions_1
15NNOEsample_1isotropicsample_conditions_1
CLEANEX-PMsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks