BMRB Entry 26545

Name: Prostate associated gene 4 (PAGE4) phosphorylated
Published: 2016-10-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26545

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Prostate associated gene 4 (PAGE4) phosphorylated

Deposition date:

2015-03-20

Original release date:

2016-10-05

Authors:

He, Yanan; Chen, Yihong; Mooney, Steven; Rajagopalan, Krithika; Bhargava, Ajay; Sacho, Elizabeth; Weninger, Keith; Kulkarni, Prakash; Orban, John

Citation:

Citation: He, Yanan; Chen, Yihong; Mooney, Steven; Rajagopalan, Krithika; Bhargava, Ajay; Sacho, Elizabeth; Weninger, Keith; Bryan, Philip; Kulkarni, Prakash; Orban, John. "Phosphorylation-induced Conformational Ensemble Switching in an Intrinsically Disordered Cancer/Testis Antigen" J. Biol. Chem. 290, 25090-25102 (2015).
PubMed: 26242913

Assembly members:

Assembly members:
Prostate_associated_gene_4_phosphorylated, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: bacterial over-expression Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Prostate_associated_gene_4_phosphorylated: MSARVRSRSRGRGDGQEAPD VVAFVAPGESQQEEPPTDNQ DIEPGQEREGXPPIEERKVE GDCQEMDLEKTRSERGDGSD VKEKTPPNPKHAKTKEAGDG QP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	353
15N chemical shifts	88
1H chemical shifts	399

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Prostate associated gene 4 phosphorylated	1

Entities:

Entity 1, Prostate associated gene 4 phosphorylated 102 residues - Formula weight is not available

1

MET

SER

ALA

ARG

VAL

ARG

SER

ARG

SER

ARG

2

GLY

ARG

GLY

ASP

GLY

GLN

GLU

ALA

PRO

ASP

3

VAL

ALA

PHE

VAL

ALA

PRO

GLY

GLU

SER

4

GLN

GLU

PRO

THR

ASP

ASN

GLN

5

ASP

ILE

GLU

PRO

GLY

GLN

GLU

ARG

GLU

GLY

6

TPO

PRO

ILE

GLU

ARG

LYS

VAL

GLU

7

GLY

ASP

CYS

GLN

GLU

MET

ASP

LEU

GLU

LYS

8

THR

ARG

SER

GLU

ARG

GLY

ASP

GLY

SER

ASP

9

VAL

LYS

GLU

LYS

THR

PRO

ASN

PRO

LYS

10

HIS

ALA

LYS

THR

LYS

GLU

ALA

GLY

ASP

GLY

11

GLN

PRO

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 200 mM; potassium phosphate 100 mM; D2O 10%; H2O 90%; NaN3 10 mM; DTT 0.1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1
15NNOE	sample_1	isotropic	sample_conditions_1
CLEANEX-PM	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks