BMRB Entry 26543

Title:
Type III Secretion Protein
Deposition date:
2015-03-20
Original release date:
2015-05-22
Authors:
Notti, Ryan; Bhattacharya, Shibani; Stebbins, Charles
Citation:

Citation: Notti, Ryan; Bhattacharya, Shibani; Lilic, Mirjana; Stebbins, Charles. "A common assembly module in injectisome and flagellar type III secretion sorting platforms"  Nat. Commun. 6, 7125-7125 (2015).
PubMed: 25994170

Assembly members:

Assembly members:
SpaO, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Salmonella enterica   Taxonomy ID: 28901   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCDFduet

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts150
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SpaO1

Entities:

Entity 1, SpaO 162 residues - Formula weight is not available

1   GLYPROVALASPVALGLYGLYGLYARGPRO
2   LYSMETLEUARGTRPPROLEUARGPHEVAL
3   ILEGLYSERSERASPTHRGLNARGSERLEU
4   LEUGLYARGILEGLYILEGLYASPVALLEU
5   LEUILEARGTHRSERARGALAGLUVALTYR
6   CYSTYRALALYSLYSLEUGLYHISPHEASN
7   ARGVALGLUGLYGLYILEILEVALGLUTHR
8   LEUASPILEGLNHISILEGLUGLUGLUASN
9   ASNTHRTHRGLUTHRALAGLUTHRLEUPRO
10   GLYLEUASNGLNLEUPROVALLYSLEUGLU
11   PHEVALLEUTYRARGLYSASNVALTHRLEU
12   ALAGLULEUGLUALAMETGLYGLNGLNGLN
13   LEULEUSERLEUPROTHRASNALAGLULEU
14   ASNVALGLUILEMETALAASNGLYVALLEU
15   LEUGLYASNGLYGLULEUVALGLNMETASN
16   ASPTHRLEUGLYVALGLUILEHISGLUTRP
17   LEUSER

Samples:

sample_1: SpaO, [U-13C; U-15N; U-2H], 0.3 mM; Sodium Citrate 10 mM; sodium chloride 100 mM; DTT 1 mM

sample_condition_1: pH: 5.6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 15N TROSYsample_1isotropicsample_condition_1
3D trHNCACBsample_1isotropicsample_condition_1
3D trHN(CO)CACBsample_1isotropicsample_condition_1
3D trHNCOsample_1isotropicsample_condition_1
3D trHNCAsample_1isotropicsample_condition_1
3D trHN(CO)CAsample_1isotropicsample_condition_1
3D trHN(CA)COsample_1isotropicsample_condition_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.5, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks