BMRB Entry 26531

Name: Human Eosinophil-Derived Neurotoxin
Published: 2015-04-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26531

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human Eosinophil-Derived Neurotoxin

Deposition date:

2015-03-08

Original release date:

2015-04-10

Authors:

Gagne, Donald; Charest, Laurie-Anne; Doucet, Nicolas

Citation:

Citation: Gagne, Donald; Narayanan, Chitra; Bafna, Khushboo; Charest, Laurie-Anne; Agarwal, Pratul; Doucet, Nicolas. "1H, 13C, and 15N backbone resonance assignment of the human eosinophil-derived neurotoxin" Biomol. NMR Assignments 11, 143-149 (2017).
PubMed: 28271277

Assembly members:

Assembly members:
EDN, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 562 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EDN: KPPQFTWAQWFETQHINMTS QQCTNAMQVINNYQRRCKNQ NTFLLTTFANVVNVCGNPNM TCPSNKTRKNCHHSGSQVPL IHCNLTTPSPQNISNCRYAQ TPANMFYIVACDNRDQRRDP PQYPVVPVHLDRII

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	261
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EDN	1

Entities:

Entity 1, EDN 134 residues - Formula weight is not available

1

LYS

PRO

GLN

PHE

THR

TRP

ALA

GLN

TRP

2

PHE

GLU

THR

GLN

HIS

ILE

ASN

MET

THR

SER

3

GLN

CYS

THR

ASN

ALA

MET

GLN

VAL

ILE

4

ASN

TYR

GLN

ARG

CYS

LYS

ASN

GLN

5

ASN

THR

PHE

LEU

THR

PHE

ALA

ASN

6

VAL

ASN

VAL

CYS

GLY

ASN

PRO

ASN

MET

7

THR

CYS

PRO

SER

ASN

LYS

THR

ARG

LYS

ASN

8

CYS

HIS

SER

GLY

SER

GLN

VAL

PRO

LEU

9

ILE

HIS

CYS

ASN

LEU

THR

PRO

SER

PRO

10

GLN

ASN

ILE

SER

ASN

CYS

ARG

TYR

ALA

GLN

11

THR

PRO

ALA

ASN

MET

PHE

TYR

ILE

VAL

ALA

12

CYS

ASP

ASN

ARG

ASP

GLN

ARG

ASP

PRO

13

PRO

GLN

TYR

PRO

VAL

PRO

VAL

HIS

LEU

14

ASP

ARG

ILE

Samples:

sample_1: EDN, [U-13C; U-15N], mM; ammonium acetate, [U-100% 15N], 7.5 mM; Glucose, [U-100% 13C], 10 % w/v; sodium acetate 15 mM

sample_conditions_1: ionic strength: 15 mM; pH: 5.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
2D HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CcpNmr_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 800 MHz

UNP	P10153
AlphaFold	Q9UCG7