BMRB Entry 2651

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR2651

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Title:
Site-Directed Mutagenesis and 1H NMR Spectroscopy of an Interdomain Segment in the Pyruvate Dehydrogenase Multienzyme Complex of Escherichia coli
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Texter, Frieda; Radford, Sheena; Laue, Ernest; Perham, Richard; Miles, John; Guest, John
Citation:

Citation: Texter, Frieda; Radford, Sheena; Laue, Ernest; Perham, Richard; Miles, John; Guest, John. "Site-Directed Mutagenesis and 1H NMR Spectroscopy of an Interdomain Segment in the Pyruvate Dehydrogenase Multienzyme Complex of Escherichia coli"  Biochemistry 27, 289-296 (1988).

Assembly members:

Assembly members:
lipoate acetyltransferase, polymer, 424 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: not available   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lipoate acetyltransferase: AIEIKVPDIGADEVEITEIL VKVGDKVEAEQSLITVEGDK ASMEVPAPFAGVVKELKVNV GDKVKTGSLIMIFEVEGAAP AAAPAKHEAAAPAPAAKAEA PAAAPAAKAEGKSEFAENDA YVHATPLIRRLAREFGVNLA KVKGTGRKGRILREDVQAYV KEAIKRAEAAPAATGGGIPG MLPWPKVDFSKFGEIEEVEL GRIQKISGANLSRNWVMIPH VTHFDKTDITELEAFRKQQN EEAAKRKLDVKITPVVFIMK AVAAALEQMPRFNSSLSEDG QRLTLKKYINIGVAVDTPNG LVVPVFKDVNKKGIIELSRE LMTISKKARDGKLTAGEMQG GCFTISSGGLGTTHFAPIVN APEVAILGVSKSAMEPVWNG KEFVPRLMLPISLSFDHRVI DGADGARFITIINNTLSDIR RLVM

Data sets:
Data typeCount
1H chemical shifts2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lipoate acetyltransferase1

Entities:

Entity 1, lipoate acetyltransferase 424 residues - Formula weight is not available

1   ALAILEGLUILELYSVALPROASPILEGLY
2   ALAASPGLUVALGLUILETHRGLUILELEU
3   VALLYSVALGLYASPLYSVALGLUALAGLU
4   GLNSERLEUILETHRVALGLUGLYASPLYS
5   ALASERMETGLUVALPROALAPROPHEALA
6   GLYVALVALLYSGLULEULYSVALASNVAL
7   GLYASPLYSVALLYSTHRGLYSERLEUILE
8   METILEPHEGLUVALGLUGLYALAALAPRO
9   ALAALAALAPROALALYSHISGLUALAALA
10   ALAPROALAPROALAALALYSALAGLUALA
11   PROALAALAALAPROALAALALYSALAGLU
12   GLYLYSSERGLUPHEALAGLUASNASPALA
13   TYRVALHISALATHRPROLEUILEARGARG
14   LEUALAARGGLUPHEGLYVALASNLEUALA
15   LYSVALLYSGLYTHRGLYARGLYSGLYARG
16   ILELEUARGGLUASPVALGLNALATYRVAL
17   LYSGLUALAILELYSARGALAGLUALAALA
18   PROALAALATHRGLYGLYGLYILEPROGLY
19   METLEUPROTRPPROLYSVALASPPHESER
20   LYSPHEGLYGLUILEGLUGLUVALGLULEU
21   GLYARGILEGLNLYSILESERGLYALAASN
22   LEUSERARGASNTRPVALMETILEPROHIS
23   VALTHRHISPHEASPLYSTHRASPILETHR
24   GLULEUGLUALAPHEARGLYSGLNGLNASN
25   GLUGLUALAALALYSARGLYSLEUASPVAL
26   LYSILETHRPROVALVALPHEILEMETLYS
27   ALAVALALAALAALALEUGLUGLNMETPRO
28   ARGPHEASNSERSERLEUSERGLUASPGLY
29   GLNARGLEUTHRLEULYSLYSTYRILEASN
30   ILEGLYVALALAVALASPTHRPROASNGLY
31   LEUVALVALPROVALPHELYSASPVALASN
32   LYSLYSGLYILEILEGLULEUSERARGGLU
33   LEUMETTHRILESERLYSLYSALAARGASP
34   GLYLYSLEUTHRALAGLYGLUMETGLNGLY
35   GLYCYSPHETHRILESERSERGLYGLYLEU
36   GLYTHRTHRHISPHEALAPROILEVALASN
37   ALAPROGLUVALALAILELEUGLYVALSER
38   LYSSERALAMETGLUPROVALTRPASNGLY
39   LYSGLUPHEVALPROARGLEUMETLEUPRO
40   ILESERLEUSERPHEASPHISARGVALILE
41   ASPGLYALAASPGLYALAARGPHEILETHR
42   ILEILEASNASNTHRLEUSERASPILEARG
43   ARGLEUVALMET

Samples:

sample_one:

sample_condition_set_one: pH: 7 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
EMBL CEE08973 CSE31214 CSE76615 CSE88811 CSE95192
GB AIW88354 EFI22553 EFW65012 EFZ46147 EGB41640
REF WP_000295776 WP_000374927 WP_032206848 WP_032230881 WP_050541395