BMRB Entry 26505

Title:
13C NMR Relaxation Studies of RNA Base and Ribose Nuclei Reveal a Complex Pattern of Motions in the RNA Binding Site for Human U1A Protein
Deposition date:
2015-01-22
Original release date:
2015-02-11
Authors:
Shajani, Zahra; Varani, Gabriele
Citation:

Citation: Shajani, Zahra; Varani, Gabriele. "13C NMR Relaxation Studies of RNA Base and Ribose Nuclei Reveal a Complex Pattern of Motions in the RNA"  J. Mol. Biol. 349, 699-715 (2005).
PubMed: 15890361

Assembly members:

Assembly members:
3'-UTR, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
3'-UTR: GGCAGAGUCCUUCGGGACAU UGCACCUGCC

Data typeCount
heteronuclear NOE values97
T1 relaxation values98
T1rho relaxation values98
order parameters65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13'-untranslated region1

Entities:

Entity 1, 3'-untranslated region 30 residues - Formula weight is not available

1   GGCAGAGUCC
2   UUCGGGACAU
3   UGCACCUGCC

Samples:

sample_1: 3'-UTR, [U-13C; U-15N], 0.7 mM; potassium phosphate 10 mM; EDTA .01 mM; H2O 99.9 v/v; D20 .1 v/v

sample_2: 3'-UTR, [U-13C; U-15N], 1.0 mM; potassium phosphate 10 mM; EDTA .01 mM; H2O 99.9 v/v; D20 .1 v/v

sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1.0 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
13C-{1H} NOEsample_1isotropicsample_conditions_1
13C-{1H} NOEsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ModelFree, Palmer - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 750 MHz