Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26376

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: The backbone resonance assignments of human vWF A2 domain [1495-1671]
Published: 2026-03-31

Title:

The backbone resonance assignments of human vWF A2 domain [1495-1671]

Deposition date:

2026-01-08

Original release date:

2026-03-31

Authors:

Hyakumoto, Ryuichi; So, Masatomo; Furukawa, Ayako; Sugase, Kenji

Citation:

Citation: Hyakumoto, Ryuichi; So, Masatomo; Furukawa, Ayako; Morimoto, Daichi; Sugase, Kenji. "1H, 13C, and 15N backbone resonance assignments of the A2 domain of human von Willebrand factor" Biomol. NMR Assign. 20, 13-13 (2026).
PubMed: 41870674

Assembly members:

Assembly members:
human vWF [1495-1671], polymer, 177 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human vWF [1495-1671]: MVLDVAFVLEGSDKIGEADF NRSKEFMEEVIQRMDVGQDS IHVTVLQYSYMVTVEYPFSE AQSKGDILQRVREIRYQGGN RTNTGLALRYLSDHSFLVSQ GDREQAPNLVYMVTGNPASD EIKRLPGDIQVVPIGVGPNA NVQELERIGWPNAPILIQDF ETLPREAPDLVLQRCCS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	424
15N chemical shifts	155
1H chemical shifts	155

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human vWF [1495-1671]	1
2	calcium ion	2

Entities:

Entity 1, human vWF [1495-1671] 177 residues - Formula weight is not available

1

MET

VAL

LEU

ASP

VAL

ALA

PHE

VAL

LEU

GLU

2

GLY

SER

ASP

LYS

ILE

GLY

GLU

ALA

ASP

PHE

3

ASN

ARG

SER

LYS

GLU

PHE

MET

GLU

VAL

4

ILE

GLN

ARG

MET

ASP

VAL

GLY

GLN

ASP

SER

5

ILE

HIS

VAL

THR

VAL

LEU

GLN

TYR

SER

TYR

6

MET

VAL

THR

VAL

GLU

TYR

PRO

PHE

SER

GLU

7

ALA

GLN

SER

LYS

GLY

ASP

ILE

LEU

GLN

ARG

8

VAL

ARG

GLU

ILE

ARG

TYR

GLN

GLY

ASN

9

ARG

THR

ASN

THR

GLY

LEU

ALA

LEU

ARG

TYR

10

LEU

SER

ASP

HIS

SER

PHE

LEU

VAL

SER

GLN

11

GLY

ASP

ARG

GLU

GLN

ALA

PRO

ASN

LEU

VAL

12

TYR

MET

VAL

THR

GLY

ASN

PRO

ALA

SER

ASP

13

GLU

ILE

LYS

ARG

LEU

PRO

GLY

ASP

ILE

GLN

14

VAL

PRO

ILE

GLY

VAL

GLY

PRO

ASN

ALA

15

ASN

VAL

GLN

GLU

LEU

GLU

ARG

ILE

GLY

TRP

16

PRO

ASN

ALA

PRO

ILE

LEU

ILE

GLN

ASP

PHE

17

GLU

THR

LEU

PRO

ARG

GLU

ALA

PRO

ASP

LEU

18

VAL

LEU

GLN

ARG

CYS

SER

Entity 2, calcium ion - Ca - 40.078 Da.

1

CA

Samples:

sample_1: human vWF [1495-1671], [U-100% 13C; U-100% 15N], 400 uM; CaCl2 10 mM; NaCl 100 mM; TRIS 50 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 100 mM; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis Assign - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 26376