BMRB Entry 26357

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26357

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Title:
Tau353Ub
Deposition date:
2025-02-23
Original release date:
2025-05-07
Authors:
Assfalg, Michael
Citation:

Citation: Viola, Giovanna; Trivellato, Daniele; Laitaoja, Mikko; Janis, Janne; Felli, Isabella C; D'Onofrio, Mariapina; Mollica, Luca; Giachin, Gabriele; Assfalg, Michael. "Conformational signatures induced by ubiquitin modification in the amyloid-forming tau repeat domain."  Proc. Natl. Acad. Sci. U. S. A. 122, e2425831122-e2425831122 (2025).
PubMed: 40198698

Assembly members:

Assembly members:
tau4RD353C, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: not available   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tau4RD353C: MQTAPVPMPDLKNVKSKIGS TENLKHQPGGGKVQIINKKL DLSNVQSKAGSKDNIKHVPG GGSVQIVYKPVDLSKVTSKA GSLGNIHHKPGGGQVEVKSE KLDFKDRVQSCIGSLDNITH VPGGGNKKIE

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts104
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1tau4RD1

Entities:

Entity 1, tau4RD 130 residues - Formula weight is not available

1   METGLNTHRALAPROVALPROMETPROASP
2   LEULYSASNVALLYSSERLYSILEGLYSER
3   THRGLUASNLEULYSHISGLNPROGLYGLY
4   GLYLYSVALGLNILEILEASNLYSLYSLEU
5   ASPLEUSERASNVALGLNSERLYSALAGLY
6   SERLYSASPASNILELYSHISVALPROGLY
7   GLYGLYSERVALGLNILEVALTYRLYSPRO
8   VALASPLEUSERLYSVALTHRSERLYSALA
9   GLYSERLEUGLYASNILEHISHISLYSPRO
10   GLYGLYGLYGLNVALGLUVALLYSSERGLU
11   LYSLEUASPPHELYSASPARGVALGLNSER
12   CYSILEGLYSERLEUASPASNILETHRHIS
13   VALPROGLYGLYGLYASNLYSLYSILEGLU

Samples:

sample_1: tau4RD353C, [U-100% 13C; U-100% 15N], 200.0 – 500.0 uM; potassium phospate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks