BMRB Entry 26348

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26348

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Title:
1H, 13C, and 15N Chemical Shift Assignments for full length 14-3-3z
Deposition date:
2024-07-12
Original release date:
2025-09-15
Authors:
Enomoto, Shota; Kuwayama, Tomoya; Yokogawa, Mariko; Osawa, Masanori
Citation:

Citation: Enomoto, Shota; Nakatsuka, Shoichi; Kuwayama, Tomoya; Kawatsu, Kosaku; Yokogawa, Mariko; Osawa, Masanori. "NMR (1)H, (13)C, (15)N backbone resonance assignments of 14-3-3z binding region of human FOXO3a (residues 1-284)."  Biomol. NMR Assign. 18, 275-283 (2024).
PubMed: 39259470

Assembly members:

Assembly members:
14-3-3z, polymer, 250 residues, 28157 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
14-3-3z: GPLGSMDKNELVQKAKLAEQ AERYDDMAACMKSVTEQGAE LSNEERNLLSVAYKNVVGAR RSSWRVVSSIEQKTEGAEKK QQMAREYREKIETELRDICN DVLSLLEKFLIPNASQAESK VFYLKMKGDYYRYLAEVAAG DDKKGIVDQSQQAYQEAFEI SKKEMQPTHPIRLGLALNFS VFYYEILNSPEKACSLAKTA FDEAIAELDTLSEESYKDST LIMQLLRDNLTLWTSDTQGD EAEAGEGGEN

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts211
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
114-3-3z1
214-3-3z_21

Entities:

Entity 1, 14-3-3z 250 residues - 28157 Da.

1   GLYPROLEUGLYSERMETASPLYSASNGLU
2   LEUVALGLNLYSALALYSLEUALAGLUGLN
3   ALAGLUARGTYRASPASPMETALAALACYS
4   METLYSSERVALTHRGLUGLNGLYALAGLU
5   LEUSERASNGLUGLUARGASNLEULEUSER
6   VALALATYRLYSASNVALVALGLYALAARG
7   ARGSERSERTRPARGVALVALSERSERILE
8   GLUGLNLYSTHRGLUGLYALAGLULYSLYS
9   GLNGLNMETALAARGGLUTYRARGGLULYS
10   ILEGLUTHRGLULEUARGASPILECYSASN
11   ASPVALLEUSERLEULEUGLULYSPHELEU
12   ILEPROASNALASERGLNALAGLUSERLYS
13   VALPHETYRLEULYSMETLYSGLYASPTYR
14   TYRARGTYRLEUALAGLUVALALAALAGLY
15   ASPASPLYSLYSGLYILEVALASPGLNSER
16   GLNGLNALATYRGLNGLUALAPHEGLUILE
17   SERLYSLYSGLUMETGLNPROTHRHISPRO
18   ILEARGLEUGLYLEUALALEUASNPHESER
19   VALPHETYRTYRGLUILELEUASNSERPRO
20   GLULYSALACYSSERLEUALALYSTHRALA
21   PHEASPGLUALAILEALAGLULEUASPTHR
22   LEUSERGLUGLUSERTYRLYSASPSERTHR
23   LEUILEMETGLNLEULEUARGASPASNLEU
24   THRLEUTRPTHRSERASPTHRGLNGLYASP
25   GLUALAGLUALAGLYGLUGLYGLYGLUASN

Samples:

sample_1: 14-3-3z, [U-13C; U-15N; U-2H], 593 uM; DTT 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: 14-3-3z, [U-15N; U-2H], 104 uM; DTT 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

TOPSPIN - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks