BMRB Entry 26345

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for FOXO3a (1-146)
Published: 2025-09-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26345

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for FOXO3a (1-146)

Deposition date:

2024-07-12

Original release date:

2025-09-15

Authors:

Enomoto, Shota; Nakatsuka, Shoichi; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Enomoto, Shota; Nakatsuka, Shoichi; Kuwayama, Tomoya; Kawatsu, Kosaku; Yokogawa, Mariko; Osawa, Masanori. "NMR (1)H, (13)C, (15)N backbone resonance assignments of 14-3-3z binding region of human FOXO3a (residues 1-284)." Biomol. NMR Assign. 18, 275-283 (2024).
PubMed: 39259470

Assembly members:

Assembly members:
FOXO3a (1-146), polymer, 151 residues, 14714 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FOXO3a (1-146): GPLGSMAEAPASPAPLSPLE VELDPEFEPQSRPRSCTWPL QRPELQASPAKPSGETAADS MIPEEEDDEDDEDGGGRAGS AMAIGGGGGSGTLGSGLLLE DSARVLAPGGQDPGSGPATA AGGLSGGTQALLQPQQPLPP PQPGAAGGSGQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	421
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FOXO3a (1-146)	1

Entities:

Entity 1, FOXO3a (1-146) 151 residues - 14714 Da.

1

GLY

PRO

LEU

GLY

SER

MET

ALA

GLU

ALA

PRO

2

ALA

SER

PRO

ALA

PRO

LEU

SER

PRO

LEU

GLU

3

VAL

GLU

LEU

ASP

PRO

GLU

PHE

GLU

PRO

GLN

4

SER

ARG

PRO

ARG

SER

CYS

THR

TRP

PRO

LEU

5

GLN

ARG

PRO

GLU

LEU

GLN

ALA

SER

PRO

ALA

6

LYS

PRO

SER

GLY

GLU

THR

ALA

ASP

SER

7

MET

ILE

PRO

GLU

ASP

GLU

ASP

8

ASP

GLU

ASP

GLY

ARG

ALA

GLY

SER

9

ALA

MET

ALA

ILE

GLY

SER

10

GLY

THR

LEU

GLY

SER

GLY

LEU

GLU

11

ASP

SER

ALA

ARG

VAL

LEU

ALA

PRO

GLY

12

GLN

ASP

PRO

GLY

SER

GLY

PRO

ALA

THR

ALA

13

ALA

GLY

LEU

SER

GLY

THR

GLN

ALA

14

LEU

GLN

PRO

GLN

PRO

LEU

PRO

15

PRO

GLN

PRO

GLY

ALA

GLY

SER

GLY

16

GLN

Samples:

sample_1: FOXO3a (1-146), [U-13C; U-15N], 466 uM; DTT 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

TOPSPIN v4.2.0 - processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks