BMRB Entry 26344

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26344

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for FOXO3a (1-146) phosphorylated on T32
Deposition date:
2024-07-12
Original release date:
2025-09-15
Authors:
Enomoto, Shota; Nakatsuka, Shoichi; Yokogawa, Mriko; Osawa, Masanori
Citation:

Citation: Enomoto, Shota; Nakatsuka, Shoichi; Kuwayama, Tomoya; Kawatsu, Kosaku; Yokogawa, Mariko; Osawa, Masanori. "NMR (1)H, (13)C, (15)N backbone resonance assignments of 14-3-3z binding region of human FOXO3a (residues 1-284)."  Biomol. NMR Assign. 18, 275-283 (2024).
PubMed: 39259470

Assembly members:

Assembly members:
FOXO3a (1-146) phosphorylated on T32, polymer, 151 residues, 14794 Da.
The secondary signals of S30, C31, X32, W33, L35, and Q36 in FOXO3a (1-146) phosphorylated on T32, polymer, 6 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FOXO3a (1-146) phosphorylated on T32: GPLGSMAEAPASPAPLSPLE VELDPEFEPQSRPRSCXWPL QRPELQASPAKPSGETAADS MIPEEEDDEDDEDGGGRAGS AMAIGGGGGSGTLGSGLLLE DSARVLAPGGQDPGSGPATA AGGLSGGTQALLQPQQPLPP PQPGAAGGSGQ
The secondary signals of S30, C31, X32, W33, L35, and Q36 in FOXO3a (1-146) phosphorylated on T32: SCXWLQ

Data sets:
Data typeCount
13C chemical shifts406
15N chemical shifts110
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 22

Entities:

Entity 1, subunit 1 151 residues - 14794 Da.

1   GLYPROLEUGLYSERMETALAGLUALAPRO
2   ALASERPROALAPROLEUSERPROLEUGLU
3   VALGLULEUASPPROGLUPHEGLUPROGLN
4   SERARGPROARGSERCYSTPOTRPPROLEU
5   GLNARGPROGLULEUGLNALASERPROALA
6   LYSPROSERGLYGLUTHRALAALAASPSER
7   METILEPROGLUGLUGLUASPASPGLUASP
8   ASPGLUASPGLYGLYGLYARGALAGLYSER
9   ALAMETALAILEGLYGLYGLYGLYGLYSER
10   GLYTHRLEUGLYSERGLYLEULEULEUGLU
11   ASPSERALAARGVALLEUALAPROGLYGLY
12   GLNASPPROGLYSERGLYPROALATHRALA
13   ALAGLYGLYLEUSERGLYGLYTHRGLNALA
14   LEULEUGLNPROGLNGLNPROLEUPROPRO
15   PROGLNPROGLYALAALAGLYGLYSERGLY
16   GLN

Entity 2, subunit 2 6 residues - Formula weight is not available

1   SERCYSTPOTRPLEUGLN

Samples:

sample_1: FOXO3a (1-146) phosphorylated on T32, [U-100% 13C; U-100% 15N], 976 uM; DTT 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D (HN)CO(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

TOPSPIN v4.2.0 - processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks