Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26339

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone resonance assignments of HNF1B POUs
Published: 2026-03-26

Title:

Backbone resonance assignments of HNF1B POUs

Deposition date:

2023-12-09

Original release date:

2026-03-26

Authors:

Hokazono, Sayaka; Ikegami, Takahisa; Konuma, Tsuyoshi

Citation:

Citation: Hokazono, Sayaka; Imagawa, Eri; Hirano, Daishi; Ikegami, Takahisa; Oishi, Kimihiko; Konuma, Tsuyoshi. "(1)H, (13)C and (15)N backbone resonance assignments of hepatocyte nuclear factor-1-beta (HNF1b) POU(S) and POU(HD)." Biomol. NMR Assign. 18, 59-63 (2024).
PubMed: 38451454

Assembly members:

Assembly members:
HNF1B POUs, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HNF1B POUs: GPGGDDYDTPPILKELQALN TEEAAEQRAEVDRMLSEDPW RAAKMIKGYMQQHNIPQREV VDVTGLNQSHLSQHLNKGTP MKTQKRAALYTWYVRKQREI LRQFNQTVQSSGN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	103
1H chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HNF1B POUs	1

Entities:

Entity 1, HNF1B POUs 113 residues - Formula weight is not available

1

GLY

PRO

GLY

ASP

TYR

ASP

THR

PRO

2

PRO

ILE

LEU

LYS

GLU

LEU

GLN

ALA

LEU

ASN

3

THR

GLU

ALA

GLU

GLN

ARG

ALA

GLU

4

VAL

ASP

ARG

MET

LEU

SER

GLU

ASP

PRO

TRP

5

ARG

ALA

LYS

MET

ILE

LYS

GLY

TYR

MET

6

GLN

HIS

ASN

ILE

PRO

GLN

ARG

GLU

VAL

7

VAL

ASP

VAL

THR

GLY

LEU

ASN

GLN

SER

HIS

8

LEU

SER

GLN

HIS

LEU

ASN

LYS

GLY

THR

PRO

9

MET

LYS

THR

GLN

LYS

ARG

ALA

LEU

TYR

10

THR

TRP

TYR

VAL

ARG

LYS

GLN

ARG

GLU

ILE

11

LEU

ARG

GLN

PHE

ASN

GLN

THR

VAL

GLN

SER

12

SER

GLY

ASN

Samples:

sample_1: HNF1B POUs, [U-95% 13C; U-90% 15N], 510 uM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

NMRViewJ - chemical shift assignment

MagRO v2.01.41 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 26339