BMRB Entry 26329

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26329

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Title:
Backbone assignment of the N terminal SH2 domain of PI3K bound to CD28 derived phosphopeptide
Deposition date:
2022-03-19
Original release date:
2023-06-05
Authors:
Hosoe, Yuhi; Miyanoiri, Yohei; Re, Suyong; Asahina, Yuya; Kawakami, Toru; Kuroda, Mataka; Mizuguchi, Kenji; Oda, Masayuki
Citation:

Citation: Hosoe, Yuhi; Miyanoiri, Yohei; Re, Suyong; Ochi, Saki; Asahina, Yuya; Kawakami, Toru; Kuroda, Masataka; Mizuguchi, Kenji; Oda, Masayuki. "Structural dynamics of the N-terminal SH2 domain of PI3K in its free and CD28-bound states"  FEBS J. 290, 2366-2378 (2023).
PubMed: 36282120

Assembly members:

Assembly members:
N terminal SH2 domain of PI3K, polymer, 107 residues, Formula weight is not available
CD28 derived phosphopeptide, polymer, 12 residues, 1501.584 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N terminal SH2 domain of PI3K: SNMSLQNAEWYWGDISREEV NEKLRDTADGTFLVRDASTK MHGDYTLTLRKGGNNKLIKI FHRDGKYGFSDPLTFSSVVE LINHYRNESLAQYNPKLDVK LLYPVSK
CD28 derived phosphopeptide: SDXMNMTPRRPG

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1nSH21
2CD28 derived phosphopeptide2

Entities:

Entity 1, nSH2 107 residues - Formula weight is not available

1   SERASNMETSERLEUGLNASNALAGLUTRP
2   TYRTRPGLYASPILESERARGGLUGLUVAL
3   ASNGLULYSLEUARGASPTHRALAASPGLY
4   THRPHELEUVALARGASPALASERTHRLYS
5   METHISGLYASPTYRTHRLEUTHRLEUARG
6   LYSGLYGLYASNASNLYSLEUILELYSILE
7   PHEHISARGASPGLYLYSTYRGLYPHESER
8   ASPPROLEUTHRPHESERSERVALVALGLU
9   LEUILEASNHISTYRARGASNGLUSERLEU
10   ALAGLNTYRASNPROLYSLEUASPVALLYS
11   LEULEUTYRPROVALSERLYS

Entity 2, CD28 derived phosphopeptide 12 residues - 1501.584 Da.

1   SERASPPTRMETASNMETTHRPROARGARG
2   PROGLY

Related Database Links:

UNP P10747

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks