BMRB Entry 26321

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26321

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Title:
Backbone resonance assignment of a LysM domain of a chitinase-A from Equisetum arvense
Deposition date:
2021-10-05
Original release date:
2022-06-16
Authors:
Kitaoku, Yoshihito; Ohnuma, Takayuki; Fukamizo, Tamo
Citation:

Citation: Kitaoku, Yoshihito; Taira, Toki; Numata, Tomoyuki; Ohnuma, Takayuki; Fukamizo, Tamo. "Structure, mechanism, and phylogeny of LysM-chitinase conjugates specifically found in fern plants."  Plant Sci. 321, 111310-. (2022).
PubMed: 35696910

Assembly members:

Assembly members:
LysM, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 3258   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Equisetum arvense

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETBlue-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LysM: ACTSYYTVKSGDICYNIAQT YGIDVATLQSYNPGLQCDNL QIGQQLCVAD

Data sets:
Data typeCount
13C chemical shifts146
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LysM1

Entities:

Entity 1, LysM 50 residues - Formula weight is not available

1   ALACYSTHRSERTYRTYRTHRVALLYSSER
2   GLYASPILECYSTYRASNILEALAGLNTHR
3   TYRGLYILEASPVALALATHRLEUGLNSER
4   TYRASNPROGLYLEUGLNCYSASPASNLEU
5   GLNILEGLYGLNGLNLEUCYSVALALAASP

Samples:

sample_1: LysM, [U-99% 13C; U-99% 15N], 151 uM; sodium acetate 20 mM; H2O 90%; D2O, U-2H, 10%

sample_conditions_1: ionic strength: 20 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - data analysis

NMRDraw - data analysis

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks