BMRB Entry 26317

Name: Backbone 1H, 13C, 15N, HA and Side Chain 13C Chemical Shift Assignments for RRM3 of PABPC1
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26317

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N, HA and Side Chain 13C Chemical Shift Assignments for RRM3 of PABPC1

Deposition date:

2021-09-07

Original release date:

2022-05-11

Authors:

Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Sunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
RRM3 of PABPC1, polymer, 104 residues, 11863 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM3 of PABPC1: GPLGSTNVYIKNFGEDMDDE RLKDLFGKFGPALSVKVMTD ESGKSKGFGFVSFERHEDAQ KAVDEMNGKELNGKQIYVGR AQKKVERQTELKRKFEQMKQ DRIT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	97
1H chemical shifts	188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 104 residues - 11863 Da.

1

GLY

PRO

LEU

GLY

SER

THR

ASN

VAL

TYR

ILE

2

LYS

ASN

PHE

GLY

GLU

ASP

MET

ASP

GLU

3

ARG

LEU

LYS

ASP

LEU

PHE

GLY

LYS

PHE

GLY

4

PRO

ALA

LEU

SER

VAL

LYS

VAL

MET

THR

ASP

5

GLU

SER

GLY

LYS

SER

LYS

GLY

PHE

GLY

PHE

6

VAL

SER

PHE

GLU

ARG

HIS

GLU

ASP

ALA

GLN

7

LYS

ALA

VAL

ASP

GLU

MET

ASN

GLY

LYS

GLU

8

LEU

ASN

GLY

LYS

GLN

ILE

TYR

VAL

GLY

ARG

9

ALA

GLN

LYS

VAL

GLU

ARG

GLN

THR

GLU

10

LEU

LYS

ARG

LYS

PHE

GLU

GLN

MET

LYS

GLN

11

ASP

ARG

ILE

THR

Samples:

sample_1: RRM3 of PABPC1, [U-100% 13C; U-100% 15N], 431 uM; sodium phosphate 18 mM; sodium chloride 135 mM; H2O 50 M; D2O, [U-2H], 10%; DTT 0.9 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks