BMRB Entry 26315

Name: Backbone 1H, 15N and HA Chemical Shift Assignments for Paip2A(25-83)
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26315

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N and HA Chemical Shift Assignments for Paip2A(25-83)

Deposition date:

2021-09-07

Original release date:

2022-05-11

Authors:

Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
Paip2A(25-83), polymer, 64 residues, 7995 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Paip2A(25-83): GPLGSHEDDNPFAEYMWMEN EEEFNRQIEEELWEEEFIER CFQEMLEEEEEHEWFIPARD LPQT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	47
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 64 residues - 7995 Da.

1

GLY

PRO

LEU

GLY

SER

HIS

GLU

ASP

ASN

2

PRO

PHE

ALA

GLU

TYR

MET

TRP

MET

GLU

ASN

3

GLU

PHE

ASN

ARG

GLN

ILE

GLU

4

GLU

LEU

TRP

GLU

PHE

ILE

GLU

ARG

5

CYS

PHE

GLN

GLU

MET

LEU

GLU

6

GLU

HIS

GLU

TRP

PHE

ILE

PRO

ALA

ARG

ASP

7

LEU

PRO

GLN

THR

Samples:

sample_1: Paip2A(25-83), [U-100% 15N], 90 uM; potassium phosphate 18 mM; sodium chloride 90 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks