BMRB Entry 26301

Title:
Crystal structure of higher plant heme oxygenase-1 and the mechanism of interaction with ferredoxin
Deposition date:
2020-10-05
Original release date:
2020-12-22
Authors:
Makiyama, Yuki; Konuma, Tsuyoshi; Ikegami, Takahisa
Citation:

Citation: Tohda, Rei; Tanaka, Hideaki; Mutoh, Risa; Zhang, Xuhong; Lee, Young-Ho; Konuma, Tsuyoshi; Ikegami, Takahisa; Migita, Taiko; Kurisu, Genji. "Crystal structure of higher plant heme oxygenase-1 and its mechanism of interaction with ferredoxin"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
ferredoxin maize, polymer, 97 residues, Formula weight is not available
FE2/S2 (INORGANIC) CLUSTER, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: Zea mays   Taxonomy ID: 4577   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Zea mays

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK233-2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts210
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fd1
2FES2

Entities:

Entity 1, Fd 97 residues - Formula weight is not available

1   ALAVALTYRLYSVALLYSLEUVALGLYPRO
2   GLUGLYGLUGLUHISGLUPHEASPALAPRO
3   ASPASPALATYRILELEUASPALAALAGLU
4   THRALAGLYVALGLULEUPROTYRSERCYS
5   ARGALAGLYALACYSSERTHRCYSALAGLY
6   LYSILEGLUSERGLYSERVALASPGLNSER
7   ASPGLYSERPHELEUASPASPGLYGLNGLN
8   GLUGLUGLYTYRVALLEUTHRCYSVALSER
9   TYRPROLYSSERASPCYSVALILEHISTHR
10   HISLYSGLUGLYASPLEUTYR

Entity 2, FES - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: FdIII, [U-13C; U-15N], 0.5 mM; FES 0.5 mM; potassium phosphate 50 mM; potassium chloride 10 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - peak picking

NMR spectrometers:

  • Bruker AVANCE III 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks