BMRB Entry 26300

Title:
The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata
Deposition date:
2020-10-01
Original release date:
2020-12-22
Authors:
Kamata, Kenichi; Mizutani, Kenji; Takahashi, Katsuya; Marchetti, Roberta; Silipo, Alba; Addy, Christine; Park, Sam-Yong; Fujii, Yuki; Fujita, Hideaki; Konuma, Tsuyoshi; Ikegami, Takahisa; Ozeki, Yasuhiro; Tame, Jeremy
Citation:

Citation: Kamata, Kenichi; Mizutani, Kenji; Takahashi, Katsuya; Marchetti, Roberta; Silipo, Alba; Addy, Christine; Park, Sam-Yong; Fujii, Yuki; Fujita, Hideaki; Konuma, Tsuyoshi; Ikegami, Takahisa; Ozeki, Yasuhiro; Tame, Jeremy. "The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata"  Sci. Rep. 10, 22102-22102 (2020).
PubMed: 33328520

Assembly members:

Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 2547956   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mytilisepta virgata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts114
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 132 residues - Formula weight is not available

The three N-terminal residues, GSH, represent the remaining non-native residues after cleaving the His-tag. The sequence number 1 starts with Met.

1   GLYSERHISMETSERSERVALTHRILEGLY
2   LYSCYSTYRILEGLNASNARGGLUASNGLY
3   GLYARGALAPHETYRASNLEUGLYARGLYS
4   ASPLEUGLYILEPHETHRGLYLYSMETTYR
5   ASPASPGLNILETRPSERPHEGLNLYSSER
6   ASPTHRPROGLYTYRTYRTHRILEGLYARG
7   GLUSERLYSPHELEUGLNTYRASNGLYGLU
8   GLNVALILEMETSERASPILEGLUGLNASP
9   THRTHRLEUTRPSERLEUGLUGLUVALPRO
10   GLUASPLYSGLYPHETYRARGLEULEUASN
11   LYSVALHISLYSALATYRLEUASPTYRASN
12   GLYGLYASPLEUVALALAASNLYSHISGLN
13   THRGLUSERGLULYSTRPILELEUPHELYS
14   ALATYR

Samples:

sample_1: SeviL dimer, [U-13C; U-15N; U-2H], 0.36 mM; sodium phosphate 50 mM; sodium chloride 20 mM; H2O 93%; D2O, [U-2H], 7%

sample_conditions_1: ionic strength: 0.07 M; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACB-trosysample_1isotropicsample_conditions_1
3D HNCOCACB-trosysample_1isotropicsample_conditions_1
3D HNCA-trosysample_1isotropicsample_conditions_1
3D HNCOCA-trosysample_1isotropicsample_conditions_1
3D HNCO-trosysample_1isotropicsample_conditions_1
3D HNCACO-trosysample_1isotropicsample_conditions_1
2D 1H-15N HSQC-trosysample_1isotropicsample_conditions_1

Software:

MagRO - peak picking

NMR spectrometers:

  • Bruker AVANCE III 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks