BMRB Entry 26069

Title:
Toward the real-time monitoring of HPV-16 E7 phosphorylation events
Deposition date:
2016-09-14
Original release date:
2017-07-05
Authors:
Nogueira, Marcela; Hosek, Tomas; Calcada, Eduardo; Castiglia, Francesca; Massimi, Paola; Banks, Lawrence; Felli, Isabella; Pierattelli, Roberta
Citation:

Citation: Nogueira, Marcela; Hosek, Tomas; Calcada, Eduardo; Castiglia, Francesca; Massimi, Paola; Banks, Lawrence; Felli, Isabella; Pierattelli, Roberta. "Monitoring HPV-16 E7 phosphorylation events."  Virology 503, 70-75 (2017).
PubMed: 28126639

Assembly members:

Assembly members:
E7_CR3, polymer, 53 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: viruses   Taxonomy ID: 10566   Superkingdom: Viruses   Kingdom: not available   Genus/species: Human papillomavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts107
15N chemical shifts37
1H chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HPV 16 E7 CR31
2ZINC ION2

Entities:

Entity 1, HPV 16 E7 CR3 53 residues - Formula weight is not available

1   ALAGLUPROASPARGALAHISTYRASNILE
2   VALTHRPHECYSCYSLYSCYSASPSERTHR
3   LEUARGLEUCYSVALGLNSERTHRHISVAL
4   ASPILEARGTHRLEUGLUASPLEULEUMET
5   GLYTHRLEUGLYILEVALCYSPROILECYS
6   SERGLNLYS

Entity 2, ZINC ION - 65.409 Da.

1   ZN

Samples:

sample_1: E7 CR3, [U-100% 13C; U-100% 15N], 0.25 mM; HEPES 50 mM; potassium chloride 50 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 8.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.0 and 3.1, Bruker Biospin - collection

ccpNMR vv2.3.1, CCPN - chemical shift assignment, data analysis, peak picking

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks