BMRB Entry 26058

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26058

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for a 58 truncated variant of the CI repressor from the temperated bacteriophage Tp901-1
Deposition date:
2016-05-18
Original release date:
2016-06-21
Authors:
Krighaar Rasmussen, Kim; Ringkjobing-Jensen, Malene
Citation:

Citation: Rasmussen, Kim; Frandsen, Kristian; Erba, Elisabetta; Pedersen, Margit; Varming, Anders; Hammer, Karin; Kilstrup, Mogens; Thulstrup, Peter; Blackledge, Martin; Jensen, Malene; Leggio, Leila. "Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1"  Sci. Rep. 6, 29574-29574 (2016).
PubMed: 27403839

Assembly members:

Assembly members:
d58CI, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lactococcus phage TP901-1   Taxonomy ID: 35345   Superkingdom: Viruses   Kingdom: not available   Genus/species: bacteriophage TP901-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
d58CI: MQTDTSNRLKQIMAERNLKQ VDILNLSIPFQKKFGIKLSK STLSQYVNSVQSPDQNRIYL LAKTLGVSEAWLMGFDVPMV ESSKIENDSENIEETITVMK KLEEPRQKVVLDTAKIQLKE QDRSHHHHHH

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dimer d58CI, chain 11
2dimer d58CI, chain 21

Entities:

Entity 1, dimer d58CI, chain 1 130 residues - Formula weight is not available

1   METGLNTHRASPTHRSERASNARGLEULYS
2   GLNILEMETALAGLUARGASNLEULYSGLN
3   VALASPILELEUASNLEUSERILEPROPHE
4   GLNLYSLYSPHEGLYILELYSLEUSERLYS
5   SERTHRLEUSERGLNTYRVALASNSERVAL
6   GLNSERPROASPGLNASNARGILETYRLEU
7   LEUALALYSTHRLEUGLYVALSERGLUALA
8   TRPLEUMETGLYPHEASPVALPROMETVAL
9   GLUSERSERLYSILEGLUASNASPSERGLU
10   ASNILEGLUGLUTHRILETHRVALMETLYS
11   LYSLEUGLUGLUPROARGGLNLYSVALVAL
12   LEUASPTHRALALYSILEGLNLEULYSGLU
13   GLNASPARGSERHISHISHISHISHISHIS

Samples:

sample_1: d58CI, [U-13C; U-15N], 500 uM; TRIS 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian Unity 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks