BMRB Entry 26044

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26044

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Title:
1HN, 13C, and 15N Resonance Assignments of the CDTb-Interacting Domain (CDTaBID) from the Clostridium difficile Binary Toxin Catalytic Component (CDTa, residues 1-216)
Deposition date:
2016-04-22
Original release date:
2016-10-14
Authors:
Roth, Braden; Weber, David; Varney, Kristen; Rustandi, Richard
Citation:

Citation: Roth, Braden; Varney, Kristen; Rustandi, Richard; Weber, David. "(1)H(N), (13)C, and (15)N resonance assignments of the CDTb-interacting domain (CDTaBID) from the Clostridium difficile binary toxin catalytic component (CDTa, residues 1-221)"  Biomol. NMR Assign. 10, 335-339 (2016).
PubMed: 27351891

Assembly members:

Assembly members:
D1_CDTa, polymer, 222 residues, 26318.8244 Da.

Natural source:

Natural source:   Common Name: Clostridium difficile   Taxonomy ID: 1496   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium difficile

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKan-SUMO-nMSD1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D1_CDTa: MVANTTYKAPIERPEDFLKD KEKAKEWERKEAERIEQKLE RSEKEALESYKKDSVEISKY SQTRNYFYDYQIEANSREKE YKELRNAISKNKIDKPMYVY YFESPEKFAFNKVIRTENQN EISLEKFNEFKETIQNKLFK QDGFKDISLYEPGKGDEKPT PLLMHLKLPRNTGMLPYTNT NNVSTLIEQGYSIKIDKIVR IVIDGKHYIKAEASVVSSLD FK

Data typeCount
13C chemical shifts642
15N chemical shifts201
1H chemical shifts201

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1D1 CDTa1

Entities:

Entity 1, D1 CDTa 222 residues - 26318.8244 Da.

1   METVALALAASNTHRTHRTYRLYSALAPRO
2   ILEGLUARGPROGLUASPPHELEULYSASP
3   LYSGLULYSALALYSGLUTRPGLUARGLYS
4   GLUALAGLUARGILEGLUGLNLYSLEUGLU
5   ARGSERGLULYSGLUALALEUGLUSERTYR
6   LYSLYSASPSERVALGLUILESERLYSTYR
7   SERGLNTHRARGASNTYRPHETYRASPTYR
8   GLNILEGLUALAASNSERARGGLULYSGLU
9   TYRLYSGLULEUARGASNALAILESERLYS
10   ASNLYSILEASPLYSPROMETTYRVALTYR
11   TYRPHEGLUSERPROGLULYSPHEALAPHE
12   ASNLYSVALILEARGTHRGLUASNGLNASN
13   GLUILESERLEUGLULYSPHEASNGLUPHE
14   LYSGLUTHRILEGLNASNLYSLEUPHELYS
15   GLNASPGLYPHELYSASPILESERLEUTYR
16   GLUPROGLYLYSGLYASPGLULYSPROTHR
17   PROLEULEUMETHISLEULYSLEUPROARG
18   ASNTHRGLYMETLEUPROTYRTHRASNTHR
19   ASNASNVALSERTHRLEUILEGLUGLNGLY
20   TYRSERILELYSILEASPLYSILEVALARG
21   ILEVALILEASPGLYLYSHISTYRILELYS
22   ALAGLUALASERVALVALSERSERLEUASP
23   PHELYS

Related Database Links:

UniProt Q9KH42
AlphaFold Q9KH42

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks