BMRB Entry 26022

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR26022
MolProbity Validation Chart

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Title:
Solution structure of translation initiation factor IF1 from wolbachia endosymbiont strain TRS of Brugia malayi
Deposition date:
2016-03-31
Original release date:
2017-03-30
Authors:
Kabra, Ashish; Nag, Jitendra; Arora, Ashish; Bhattacharya, Shailja
Citation:

Citation: Kabra, Ashish; Nag, Jitendra; Arora, Ashish; Bhattacharya, Shailja. "Solution structure of translation initiation factor IF1 from wolbachia endosymbiont strain TRS of Brugia malayi"  .

Assembly members:

Assembly members:
IF1, polymer, 87 residues, 9956.837 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 953   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IF1: MVKDEKSKTLFEVEGAVTAL LPAAEFRVKLDNEHEIICHV SGKVRRSKIRIIIGDRVLVE MSIYDRNAKKGRIIRRLKGT SDRTISK

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts83
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1translation initiation factor IF11

Entities:

Entity 1, translation initiation factor IF1 87 residues - 9956.837 Da.

Sequence does not contain the purification tag.

1   METVALLYSASPGLULYSSERLYSTHRLEU
2   PHEGLUVALGLUGLYALAVALTHRALALEU
3   LEUPROALAALAGLUPHEARGVALLYSLEU
4   ASPASNGLUHISGLUILEILECYSHISVAL
5   SERGLYLYSVALARGARGSERLYSILEARG
6   ILEILEILEGLYASPARGVALLEUVALGLU
7   METSERILETYRASPARGASNALALYSLYS
8   GLYARGILEILEARGARGLEULYSGLYTHR
9   SERASPARGTHRILESERLYS

Samples:

sample_1: IF1, [U-100% 15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; PMSF 2 mM

sample_2: IF1, [U-100% 13C; U-100% 15N], 0.8 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; PMSF 2 mM

sample_3: IF1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; PMSF 2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

PSVS, Bhattacharya and Montelione - Validation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks