BMRB Entry 25988

Name: BAZ2B PHD
Published: 2017-04-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25988

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BAZ2B PHD

Deposition date:

2016-03-10

Original release date:

2017-04-13

Authors:

Bortoluzzi, Alessio; Ciulli, Alessio

Citation:

Citation: Bortoluzzi, Alessio; Amato, Anastasia; Lucas, Xavier; Blank, Manuel; Ciulli, Alessio. "Structural Basis of Molecular Recognition of Helical Histone H3 Tail by PHD Finger Domains" Biochem J. 474, 1633-1651 (2017).
PubMed: 28341809

Assembly members:

Assembly members:
BAZ2B_PHD, polymer, 58 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BAZ2B_PHD: HMSIMKVYCQICRKGDNEEL LLLCDGCDKGCHTYCHRPKI TTIPDGDWFCPACIAKAS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	160
15N chemical shifts	53
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BAZ2B PHD	1
2	Zinc1	2
3	Zinc2	2

Entities:

Entity 1, BAZ2B PHD 58 residues - Formula weight is not available

1

HIS

MET

SER

ILE

MET

LYS

VAL

TYR

CYS

GLN

2

ILE

CYS

ARG

LYS

GLY

ASP

ASN

GLU

LEU

3

LEU

CYS

ASP

GLY

CYS

ASP

LYS

GLY

4

CYS

HIS

THR

TYR

CYS

HIS

ARG

PRO

LYS

ILE

5

THR

ILE

PRO

ASP

GLY

ASP

TRP

PHE

CYS

6

PRO

ALA

CYS

ILE

ALA

LYS

ALA

SER

Entity 2, Zinc1 - 65.409 Da.

1

ZN

Samples:

sample_1: BAZ2B_PHD, [U-98% 13C; U-98% 15N], 350 uM; DTT 1 mM; potassium chloride 50 mM; potassium phosphate 25 mM; sodium azide 0.02 w/v; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.200 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CcpNmr_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance III HD 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks