BMRB Entry 25976

Title:
Solution structure of the HYD1 hydrophobin from Schizophyllum commune
Deposition date:
2016-02-21
Original release date:
2016-03-30
Authors:
Langelaan, David; Smith, Steven; Grondin, Julie
Citation:

Citation: Langelaan, David; Smith, Steven; Master, Emma; Gandier, Julie-Anne; Grondin, Julie. "Structure of a fungal hydrophobin"  .

Assembly members:

Assembly members:
Hydrophobin, polymer, 107 residues, 10873.522 Da.

Natural source:

Natural source:   Common Name: Schizophyllum commune   Taxonomy ID: 5334   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizophyllum commune

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 32

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts91
1H chemical shifts574

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hydrophobin1

Entities:

Entity 1, Hydrophobin 107 residues - 10873.522 Da.

1   LYSALAMETALAASPILEGLYSERTHRALA
2   VALPROARGASPVALASNGLYGLYTHRPRO
3   PROLYSSERCYSSERSERGLYPROVALTYR
4   CYSCYSASNLYSTHRGLUASPSERLYSHIS
5   LEUASPLYSGLYTHRTHRALALEULEUGLY
6   LEULEUASNILELYSILEGLYASPLEULYS
7   ASPLEUVALGLYLEUASNCYSSERPROLEU
8   SERVALILEGLYVALGLYGLYASNSERCYS
9   SERALAGLNTHRVALCYSCYSTHRASNTHR
10   TYRGLNHISGLYLEUVALASNVALGLYCYS
11   THRPROILEASNILEGLYLEU

Samples:

sample_1: Hydrophobin, [U-13C; U-15N], 1 mM; TRIS 25 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
1H-15N Heteronuclear NOEsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

CcpNmr_Analysis, Wim F. Vranken, Wayne Boucher, Tim J. Stevens, Rasmus H. Fogh, - chemical shift assignment

ARIA v1.3, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP D8QCG9_SCHCM
AlphaFold D8QCG9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks