BMRB Entry 25934

Name: Nizp1-C2HR zinc finger structure
Published: 2016-02-23

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PDB ID: 2nab
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25934
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Nizp1-C2HR zinc finger structure

Deposition date:

2015-12-22

Original release date:

2016-02-23

Authors:

Berardi, Andrea; Quilici, Giacomo; Spiliotopoulos, Dimitrios; Corral-Rodriguez, Maria Angeles; Martin, Fernando; Degano, Massimo; Tonon, Giovanni; Musco, Giovanna

Citation:

Citation: Berardi, Andrea; Quilici, Giacomo; Spiliotopoulos, Dimitrios; Corral-Rodriguez, Maria Angeles; Martin, Fernando; Degano, Massimo; Tonon, Giovanni; Musco, Giovanna. "Structural basis for PHDVC5HCHNSD1-C2HRNizp1 interaction: implications for Sotos syndrome" Nucleic Acids Res. 44, 3448-3463 (2016).
PubMed: 26896805

Assembly members:

Assembly members:
entity_1, polymer, 41 residues, 4984.875 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMEVQTSQKSYVCPNCGKI FRWRVNFIRHLRSRREQKPH K

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	37
1H chemical shifts	267

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 41 residues - 4984.875 Da.

1

GLY

ALA

MET

GLU

VAL

GLN

THR

SER

GLN

LYS

2

SER

TYR

VAL

CYS

PRO

ASN

CYS

GLY

LYS

ILE

3

PHE

ARG

TRP

ARG

VAL

ASN

PHE

ILE

ARG

HIS

4

LEU

ARG

SER

ARG

GLU

GLN

LYS

PRO

HIS

5

LYS

Entity 2, ZINC ION - 65.409 Da.

1

ZN

Samples:

sample_1: sodium phosphate, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 90%; D2O 10%

sample_2: sodium phosphate, [U-99% 15N], 0.8 mM; H2O 90%; D2O 10%

sample_3: sodium phosphate 0.8 mM; H2O 90%; D2O 10%

sample_4: sodium phosphate 0.8 mM; H2O 100%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.3; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks