BMRB Entry 25909

Title:
1H, 13C, and 15N Chemical Shift Assignments for in vitro GB1
Deposition date:
2015-11-26
Original release date:
2016-12-21
Authors:
Ikeya, Teppei; Hanashima, Tomomi; Hosoya, Saori; Shimazaki, Manato; Ikeda, Shiro; Mishima, Masaki; Guentert, Peter; Ito, Yutaka
Citation:

Citation: Ikeya, Teppei; Hanashima, Tomomi; Hosoya, Saori; Shimazaki, Manato; Ikeda, Shiro; Mishima, Masaki; Guentert, Peter; Ito, Yutaka. "Improved in-cell structure determination of proteins at near-physiological concentration"  Sci. Rep. 6, 38312-38312 (2016).
PubMed: 27910948

Assembly members:

Assembly members:
entity, polymer, 57 residues, 6258.896 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET47b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts62
1H chemical shifts384

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 57 residues - 6258.896 Da.

1   METGLYTHRTYRLYSLEUILELEUASNGLY
2   LYSTHRLEULYSGLYGLUTHRTHRTHRGLU
3   ALAVALASPALAALATHRALAGLULYSVAL
4   PHELYSGLNTYRALAASNASPASNGLYVAL
5   ASPGLYGLUTRPTHRTYRASPASPALATHR
6   LYSTHRPHETHRVALTHRGLU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks