BMRB Entry 25905

Name: Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)
Published: 2016-11-17

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PDB ID: 2n9g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25905
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)

Deposition date:

2015-11-23

Original release date:

2016-11-17

Authors:

Yang, Xiao

Citation:

Citation: Yang, Xiao. "Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)" .

Assembly members:

Assembly members:
entity, polymer, 111 residues, 12723.368 Da.

Natural source:

Natural source: Common Name: kinetoplastids Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MSKNERDTSFNKNGCLVFVS RLWDLDKLGMFHHPVSAEEL PDYHTVIKRPVDLSSIRDGI EKGTYATDVDVQNDVARMIT NALEYNAKGSTWYQEAMSFR KTYLDLARQSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	292
15N chemical shifts	112
1H chemical shifts	501

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 111 residues - 12723.368 Da.

1

MET

SER

LYS

ASN

GLU

ARG

ASP

THR

SER

PHE

2

ASN

LYS

ASN

GLY

CYS

LEU

VAL

PHE

VAL

SER

3

ARG

LEU

TRP

ASP

LEU

ASP

LYS

LEU

GLY

MET

4

PHE

HIS

PRO

VAL

SER

ALA

GLU

LEU

5

PRO

ASP

TYR

HIS

THR

VAL

ILE

LYS

ARG

PRO

6

VAL

ASP

LEU

SER

ILE

ARG

ASP

GLY

ILE

7

GLU

LYS

GLY

THR

TYR

ALA

THR

ASP

VAL

ASP

8

VAL

GLN

ASN

ASP

VAL

ALA

ARG

MET

ILE

THR

9

ASN

ALA

LEU

GLU

TYR

ASN

ALA

LYS

GLY

SER

10

THR

TRP

TYR

GLN

GLU

ALA

MET

SER

PHE

ARG

11

LYS

THR

TYR

LEU

ASP

LEU

ALA

ARG

GLN

SER

12

GLY

Samples:

sample_1: entity 0.5 mM; sodium chloride 150 mM; sodium phosphate 20 mM; DTT 1 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker DMX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks