BMRB Entry 25900

Name: NRAS Isoform 5
Published: 2016-03-21

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PDB ID: 2n9c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25900
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NRAS Isoform 5

Deposition date:

2015-11-13

Original release date:

2016-03-21

Authors:

Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William

Citation:

Citation: Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William. "Structural Characterization of NRAS Isoform 5" Protein Sci. 25, 1069-1074 (2016).
PubMed: 26947772

Assembly members:

Assembly members:
entity, polymer, 20 residues, 2120.511 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MTEYKLVVVGAGGVGKSHVW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	150

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 20 residues - 2120.511 Da.

1		MET	THR	GLU	TYR	LYS	LEU	VAL	VAL	VAL	GLY
2		ALA	GLY	GLY	VAL	GLY	LYS	SER	HIS	VAL	TRP

Samples:

sample_1: TFE, [U-99% 2H], 56%; entity 1 mM; sodium azide 10 uM; sodium phosphate 4 mM; EDTA, [U-99% 2H], 50 uM

sample_conditions_1: ionic strength: 0.004 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 13C-1H HSQC-TOCSY	sample_1	isotropic	sample_conditions_1
2D 13C-1H HSQC-NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

1		MET	THR	GLU	TYR	LYS	LEU	VAL	VAL	VAL	GLY
2		ALA	GLY	GLY	VAL	GLY	LYS	SER	HIS	VAL	TRP

1		MET	THR	GLU	TYR	LYS	LEU	VAL	VAL	VAL	GLY
2		ALA	GLY	GLY	VAL	GLY	LYS	SER	HIS	VAL	TRP

1		MET	THR	GLU	TYR	LYS	LEU	VAL	VAL	VAL	GLY
2		ALA	GLY	GLY	VAL	GLY	LYS	SER	HIS	VAL	TRP