BMRB Entry 25896

Name: Backbone Assignment of Apo Troponin C from Lethocerus Indicus
Published: 2016-01-29

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25896

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone Assignment of Apo Troponin C from Lethocerus Indicus

Deposition date:

2015-11-12

Original release date:

2016-01-29

Authors:

Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda; Adrover, Miguel

Citation:

Citation: Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda; Adrover, Miguel. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus" J. Biol. Chem. 291, 16090-16099 (2016).
PubMed: 27226601

Assembly members:

Assembly members:
apo_TnC, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Lethocerus Indicus Taxonomy ID: 212017 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lethocerus Indicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apo_TnC: MVAMDDLDKDQIALLKRAFE AFAQEKSYIEANMVGTILQM LGHEVSDKQLTEIIVEVDAD GSGQLEFEEFVTLAARFLTE DNEEDSAAMMEELREAFRLY DKDGNGYITTEVLREILKEL DDKITPEDLDMMIQEIDSDG SGTVDFDEFMEVMIGGDE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	441
15N chemical shifts	154
1H chemical shifts	154

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Apo F2 TnC	1

Entities:

Entity 1, Apo F2 TnC 158 residues - Formula weight is not available

1

MET

VAL

ALA

MET

ASP

LEU

ASP

LYS

ASP

2

GLN

ILE

ALA

LEU

LYS

ARG

ALA

PHE

GLU

3

ALA

PHE

ALA

GLN

GLU

LYS

SER

TYR

ILE

GLU

4

ALA

ASN

MET

VAL

GLY

THR

ILE

LEU

GLN

MET

5

LEU

GLY

HIS

GLU

VAL

SER

ASP

LYS

GLN

LEU

6

THR

GLU

ILE

VAL

GLU

VAL

ASP

ALA

ASP

7

GLY

SER

GLY

GLN

LEU

GLU

PHE

GLU

PHE

8

VAL

THR

LEU

ALA

ARG

PHE

LEU

THR

GLU

9

ASP

ASN

GLU

ASP

SER

ALA

MET

10

GLU

LEU

ARG

GLU

ALA

PHE

ARG

LEU

TYR

11

ASP

LYS

ASP

GLY

ASN

GLY

TYR

ILE

THR

12

GLU

VAL

LEU

ARG

GLU

ILE

LEU

LYS

GLU

LEU

13

ASP

LYS

ILE

THR

PRO

GLU

ASP

LEU

ASP

14

MET

ILE

GLN

GLU

ILE

ASP

SER

ASP

GLY

15

SER

GLY

THR

VAL

ASP

PHE

ASP

GLU

PHE

MET

16

GLU

VAL

MET

ILE

GLY

ASP

GLU

Samples:

sample_1: apo TnC, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; TRIS, [U-2H], 20 mM; KCl 100 mM; DTT, [U-2H], 2 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks