BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25896

Title: Backbone Assignment of Apo Troponin C from Lethocerus Indicus   PubMed: 27226601

Deposition date: 2015-11-12 Original release date: original

Authors: Sanfelice, Domenico; ., Pastore; ., .; ., .

Citation: Sanfelice, Domenico; ., Pastore; ., .; ., .. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus"  J. Biol. Chem. 291, 16090-16099 (2016).

Assembly members:
apo_TnC, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 212017   Superkingdom: Metazoa   Kingdom: Lethocerus   Genus/species: Indicus not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
apo_TnC: MVAMDDLDKDQIALLKRAFE AFAQEKSYIEANMVGTILQM LGHEVSDKQLTEIIVEVDAD GSGQLEFEEFVTLAARFLTE DNEEDSAAMMEELREAFRLY DKDGNGYITTEVLREILKEL DDKITPEDLDMMIQEIDSDG SGTVDFDEFMEVMIGGDE

Data sets:
Data typeCount
13C chemical shifts441
1541H chemical shifts

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apo F2 TnC1

Entities:

Entity 1, Apo F2 TnC 158 residues - Formula weight is not available

1   MET.2589656LEU.258961112
2   ALA.258961718PHE.258962324
3   GLU.258962930ALA.258963536
4   ILE.258964142HIS.258964748
5   GLN.258965354VAL.258965960
6   GLY.258966566PHE.258967172
7   LEU.258967778THR.258968384
8   ASP.258968990GLU.258969596
9   PHE.25896101102ASP.25896107108
10   THR.25896113114GLU.25896119120
11   ASP.25896125126GLU.25896131132
12   ILE.25896137138ASP.25896143144
13   ASP.25896149150GLU.25896155156
14   ASP.

Samples:

sample_1: apo TnC' '[U-98% 13C; U-98% 15N] 0.5; .; . mM; .; . 25896; ., solvent,

sample_conditions_1: ionic strength: 0.1 M; 6.8: . 25896; .: atm temperature; K: 25896

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$sample_conditions_1not available
not availablesample_conditions_1not availablenot available
not availablenot availablenot availablenot available

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts