BMRB Entry 25875

Name: Backbone assignment of Calcium loaded C-terminus of Troponin C isoform 2
Published: 2016-01-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25875

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Calcium loaded C-terminus of Troponin C isoform 2

Deposition date:

2015-11-02

Original release date:

2016-01-29

Authors:

Sanfelice, Domenico; Pastore, Annalisa; Adrover, Miguel

Citation:

Citation: Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus" J. Biol. Chem. 291, 16090-16099 (2016).
PubMed: 27226601

Assembly members:

Assembly members:
Cterm_TnC_F2, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Lethocerus Indicus Taxonomy ID: 212017 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lethocerus Indicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cterm_TnC_F2: GMMEELREAFRLYDKDGNGY ITTEVLREILKELDDKITPE DLDMMIQEIDSDGSGTVDFD EFMEVMIGGDE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	205
15N chemical shifts	66
1H chemical shifts	356

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminus F2 TnC	1

Entities:

Entity 1, C-terminus F2 TnC 71 residues - Formula weight is not available

seq starts at 88

1

GLY

MET

GLU

LEU

ARG

GLU

ALA

PHE

2

ARG

LEU

TYR

ASP

LYS

ASP

GLY

ASN

GLY

TYR

3

ILE

THR

GLU

VAL

LEU

ARG

GLU

ILE

LEU

4

LYS

GLU

LEU

ASP

LYS

ILE

THR

PRO

GLU

5

ASP

LEU

ASP

MET

ILE

GLN

GLU

ILE

ASP

6

SER

ASP

GLY

SER

GLY

THR

VAL

ASP

PHE

ASP

7

GLU

PHE

MET

GLU

VAL

MET

ILE

GLY

ASP

8

GLU

Samples:

sample_1: Cterm TnC F2, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

XEASY v1.2, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks