BMRB Entry 25866

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PDB ID: 2n8u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25866
MolProbity Validation Chart

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Title:
Solution Structure of the rNedd4 WW2 Domain by NMR
Deposition date:
2015-10-27
Original release date:
2016-02-22
Authors:
Spagnol, Gaelle; Kieken, Fabien; Sorgen, Paul
Citation:

Citation: Spagnol, Gaelle; Kieken, Fabien; Kopanic, Jennifer; Li, Hanjun; Zach, Sydney; Stauch, Kelly; Grosely, Rosslyn; Sorgen, Paul. "Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus"  J. Biol. Chem. 291, 7637-7650 (2016).
PubMed: 26841867

Assembly members:

Assembly members:
rNedd4_WW2, polymer, 39 residues, 4436.736 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rNedd4_WW2: GSSSGLPPGWEEKQDDRGRS YYVDHNSKTTTWSKPTMED

Data sets:
Data typeCount
13C chemical shifts118
15N chemical shifts35
1H chemical shifts223

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1rNedd4 WW2 Domain1

Entities:

Entity 1, rNedd4 WW2 Domain 39 residues - 4436.736 Da.

1   GLYSERSERSERGLYLEUPROPROGLYTRP
2   GLUGLULYSGLNASPASPARGGLYARGSER
3   TYRTYRVALASPHISASNSERLYSTHRTHR
4   THRTRPSERLYSPROTHRMETGLUASP

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