BMRB Entry 25858

Title:
Solution Structure of Lacticin Q and Aureocin A53
Deposition date:
2015-10-22
Original release date:
2016-02-15
Authors:
Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John
Citation:

Citation: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John. "Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity"  Biochemistry 55, 733-742 (2016).
PubMed: 26771761

Assembly members:

Assembly members:
Lacticin_Q, polymer, 53 residues, 5906.144 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: Eubacteria   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Lacticin_Q: MAGFLKVVQLLAKYGSKAVQ WAWANKGKILDWLNAGQAID WVVSKIKQILGIK

Data sets:
Data typeCount
1H chemical shifts391

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lacticin Q1

Entities:

Entity 1, Lacticin Q 53 residues - 5906.144 Da.

1   METALAGLYPHELEULYSVALVALGLNLEU
2   LEUALALYSTYRGLYSERLYSALAVALGLN
3   TRPALATRPALAASNLYSGLYLYSILELEU
4   ASPTRPLEUASNALAGLYGLNALAILEASP
5   TRPVALVALSERLYSILELYSGLNILELEU
6   GLYILELYS

Samples:

sample_1: Lacticin Q 0.8 mM; DSS 0.01 % w/v; TFE, [U-99% 2H], 25%; H2O 75%

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Varian vNMRJ 700 MHz