BMRB Entry 25854

Title:
Zipcode-binding-protein-1 KH3KH4(DD) domains in complex with the RNA target CACACCC   PubMed: 20080952
Deposition date:
2015-10-21
Original release date:
2017-01-19
Authors:
Nicastro, Giuseppe; Candel, Adela; Ramos, Andres; Hollingworth, David
Citation:

Citation: Chao, Jeffrey; Patskovsky, Yury; Patel, Vivek; Levy, Matthew; Almo, Steven; Singer, Robert. "ZBP1 recognition of beta-actin zipcode induces RNA looping."  Genes Dev. 24, 148-158 (2010).

Assembly members:

Assembly members:
entity_1, polymer, 191 residues, 20685.811 Da.
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts403
1H chemical shifts941

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 191 residues - 20685.811 Da.

1   GLYALAMETGLYPROSERSERVALSERGLY
2   ALAALAPROPHESERSERPHEMETPROPRO
3   GLUGLNGLUTHRVALHISVALPHEILEPRO
4   ALAGLNALAVALGLYALAILEILEGLYLYS
5   LYSGLYGLNHISILELYSGLNLEUSERARG
6   PHEALASERALASERILELYSILEALAPRO
7   PROGLUTHRPROASPSERLYSVALARGMET
8   VALVALILETHRGLYPROPROGLUALAGLN
9   PHELYSALAGLNGLYARGILETYRGLYLYS
10   LEULYSGLUGLUASNPHEPHEGLYPROLYS
11   GLUGLUVALLYSLEUGLUTHRHISILEARG
12   VALPROALASERALAALAGLYARGVALILE
13   GLYASPASPGLYLYSTHRVALASNGLULEU
14   GLNASNLEUTHRALAALAGLUVALVALVAL
15   PROARGASPGLNTHRPROASPGLUASNGLU
16   GLNVALILEVALLYSILEILEGLYHISPHE
17   TYRALASERGLNMETALAGLNARGLYSILE
18   ARGASPILELEUALAGLNVALLYSGLNGLN
19   HISGLNLYSGLYGLNSERGLYGLNLEUGLN
20   ALA

Entity 2, entity_2 8 residues - Formula weight is not available

1   CACACCC

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.3 mM; entity_2 0.3 mM; phosphate buffer 20 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 950 MHz