BMRB Entry 25838

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal intrinsically disordered domain of Alb3
Published: 2016-01-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25838

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal intrinsically disordered domain of Alb3

Deposition date:

2015-10-06

Original release date:

2016-01-06

Authors:

Sattler, Michael; Hennig, Janosch

Citation:

Citation: Horn, Annemarie; Hennig, Janosch; Ahmed, Yasar; Stier, Gunter; Wild, Klemens; Sattler, Michael; Hennig, Janosch. "Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction" Nat. Commun. 6, 8875-8875 (2015).
PubMed: 26568381

Assembly members:

Assembly members:
Alb3-A3CT, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alb3-A3CT: MDENASKIISAGRAKRSIAQ PDDAGERFRQLKEQEKRSKK NKAVAKDTVELVEESQSESE EGSDDEEEEAREGALASSTT SKPLPEVGQRRSKRSKRKRT V

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	153
15N chemical shifts	79
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A3CT monomer	1

Entities:

Entity 1, A3CT monomer 101 residues - Formula weight is not available

1

MET

ASP

GLU

ASN

ALA

SER

LYS

ILE

SER

2

ALA

GLY

ARG

ALA

LYS

ARG

SER

ILE

ALA

GLN

3

PRO

ASP

ALA

GLY

GLU

ARG

PHE

ARG

GLN

4

LEU

LYS

GLU

GLN

GLU

LYS

ARG

SER

LYS

5

ASN

LYS

ALA

VAL

ALA

LYS

ASP

THR

VAL

GLU

6

LEU

VAL

GLU

SER

GLN

SER

GLU

SER

GLU

7

GLU

GLY

SER

ASP

GLU

ALA

8

ARG

GLU

GLY

ALA

LEU

ALA

SER

THR

9

SER

LYS

PRO

LEU

PRO

GLU

VAL

GLY

GLN

ARG

10

ARG

SER

LYS

ARG

SER

LYS

ARG

LYS

ARG

THR

11

VAL

Samples:

sample_1: Alb3-A3CT, [U-100% 13C; U-100% 15N], 0.45 mM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks