BMRB Entry 25832

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25832

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Title:
NMR assignment of CPR3 from Saccharomyces cerevisiae
Deposition date:
2015-10-02
Original release date:
2016-09-02
Authors:
Shukla, Vaibhav; Singh, Jai; Trivedi, Dipesh; Kumar, Ashutosh; Hosur, Ramakrishna V.
Citation:

Citation: Shukla, Vaibhav; Singh, Jai; Trivedi, Dipesh; Hosur, Ramakrishna V.; Kumar, Ashutosh. "NMR assignments of mitochondrial cyclophilin Cpr3 from Saccharomyces cerevisiae"  Biomol. NMR Assign. 10, 203-206 (2016).
PubMed: 26897529

Assembly members:

Assembly members:
CPR3, polymer, 182 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CPR3: MFKRSIIQQSRLFSNSASRL GKKVFFDPAVNGTKIGRIEF ELYDNVVPKTAENFRALCTG EKGWGYKGVPFHRIIPDFMI QGGDTDLTNGFGGKSIYGSK FADENFVKKHDKAGLLSMAN AGPNTNGSQFFITTVPCPWL DGKHVVFGEVTKGMDIVKAI ESYGTASGKPRAEIVIEEAG EL

Data sets:
Data typeCount
13C chemical shifts673
15N chemical shifts164
1H chemical shifts970

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CPR31

Entities:

Entity 1, CPR3 182 residues - Formula weight is not available

1   METPHELYSARGSERILEILEGLNGLNSER
2   ARGLEUPHESERASNSERALASERARGLEU
3   GLYLYSLYSVALPHEPHEASPPROALAVAL
4   ASNGLYTHRLYSILEGLYARGILEGLUPHE
5   GLULEUTYRASPASNVALVALPROLYSTHR
6   ALAGLUASNPHEARGALALEUCYSTHRGLY
7   GLULYSGLYTRPGLYTYRLYSGLYVALPRO
8   PHEHISARGILEILEPROASPPHEMETILE
9   GLNGLYGLYASPTHRASPLEUTHRASNGLY
10   PHEGLYGLYLYSSERILETYRGLYSERLYS
11   PHEALAASPGLUASNPHEVALLYSLYSHIS
12   ASPLYSALAGLYLEULEUSERMETALAASN
13   ALAGLYPROASNTHRASNGLYSERGLNPHE
14   PHEILETHRTHRVALPROCYSPROTRPLEU
15   ASPGLYLYSHISVALVALPHEGLYGLUVAL
16   THRLYSGLYMETASPILEVALLYSALAILE
17   GLUSERTYRGLYTHRALASERGLYLYSPRO
18   ARGALAGLUILEVALILEGLUGLUALAGLY
19   GLULEU

Samples:

sample_1: CPR3, [U-99% 15N], 0.8 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1.0 mM; D2O, [U-2H], 10%; H2O 90%

sample_2: CPR3, [U-99% 13C; U-99% 15N], 0.8 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1.0 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D-HN(CA)COsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D-HCCcoHNsample_2isotropicsample_conditions_1
3D-CCcoNHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Ascend 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks