BMRB Entry 25792

Name: p65dd
Published: 2016-09-02

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25792

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

p65dd

Deposition date:

2015-09-02

Original release date:

2016-09-02

Authors:

Dyson, Jane; Mukherjee, Sulakshana; Borin, Brendan; Quintas, Pedro

Citation:

Citation: Mukherjee, Sulakshana; Borin, Brendan; Quintas, Pedro; Dyson, Jane. "NMR characterization of a 72 kDa transcription factor using differential isotopic labeling" Protein Sci. 25, 597-604 (2016).
PubMed: 26647230

Assembly members:

Assembly members:
p65dd, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p65dd: NTAELKICRVNRNSGSCLGG DEIFLLCDKVQKEDIEVYFT GPGWEARGSFSQADVHRQVA IVFRTPPYADPSLQAPVRVS MQLRRPSDRELSEPMEFQYL PDTDDRHRIEEKRKRTYETF KSIMKKSPFNGP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	255
15N chemical shifts	125
1H chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p65 dimerization domain	1

Entities:

Entity 1, p65 dimerization domain 132 residues - Formula weight is not available

1

ASN

THR

ALA

GLU

LEU

LYS

ILE

CYS

ARG

VAL

2

ASN

ARG

ASN

SER

GLY

SER

CYS

LEU

GLY

3

ASP

GLU

ILE

PHE

LEU

CYS

ASP

LYS

VAL

4

GLN

LYS

GLU

ASP

ILE

GLU

VAL

TYR

PHE

THR

5

GLY

PRO

GLY

TRP

GLU

ALA

ARG

GLY

SER

PHE

6

SER

GLN

ALA

ASP

VAL

HIS

ARG

GLN

VAL

ALA

7

ILE

VAL

PHE

ARG

THR

PRO

TYR

ALA

ASP

8

PRO

SER

LEU

GLN

ALA

PRO

VAL

ARG

VAL

SER

9

MET

GLN

LEU

ARG

PRO

SER

ASP

ARG

GLU

10

LEU

SER

GLU

PRO

MET

GLU

PHE

GLN

TYR

LEU

11

PRO

ASP

THR

ASP

ARG

HIS

ARG

ILE

GLU

12

GLU

LYS

ARG

LYS

ARG

THR

TYR

GLU

THR

PHE

13

LYS

SER

ILE

MET

LYS

SER

PRO

PHE

ASN

14

GLY

PRO

Samples:

sample_1: p65dd, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 ± 0.1 mM; p50dd, [U-100% 2H], 0.3 ± 0.1 mM; TRIS, [U-2H], 20 mM; sodium chloride 50 mM; DTT, [U-2H], 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 750 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks