BMRB Entry 25777

Name: Solution NMR structure of Tetrahymena telomerase RNA pseudoknot
Published: 2015-10-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25777

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of Tetrahymena telomerase RNA pseudoknot

Deposition date:

2015-08-27

Original release date:

2015-10-26

Authors:

Cash, Darian; Feigon, Juli

Citation:

Citation: Jiang, Jiansen; Chan, Henry; Cash, Darian; Miracco, Edward; Ogorzalek Loo, Rachel; Cascio, Dulio; O'Brien Johnson, Reid; Upton, Heather; Collins, Kathleen; Loo, Joseph; Zhou, Hong; Feigon, Juli. "Structure of Tetrahymena telomerase reveals new subunits, functions, and interactions" Science 350, 4070-4070 (2015).
PubMed: 26472759

Assembly members:

Assembly members:
RNA_(31-MER), polymer, 31 residues, 9888.990 Da.

Natural source:

Natural source: Common Name: ciliates Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: in vitro transcription Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(31-MER): XACCUUCACCAAUUAGGUUC AAAUAAGUGGU

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	80
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA (31-MER)	1

Entities:

Entity 1, RNA (31-MER) 31 residues - 9888.990 Da.

1

ADN

A

C

U

C

A

C

2

A

U

A

G

U

C

3

A

U

A

G

U

G

4

U

Samples:

sample_1: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; H2O 90%; D2O 10%

sample_2_D2O: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; D2O 100%

sample_3_AU: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; ADENOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; URIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_4_GC: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; GUANOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_5_AUGC: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; GUANOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; ADENOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D imino NOESY	sample_1	isotropic	sample_conditions_1
2D D2O NOESY	sample_2_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3_AU	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4_GC	isotropic	sample_conditions_1
2D TOCSY	sample_2_D2O	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3_AU	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4_GC	isotropic	sample_conditions_1
jNN-COSY	sample_3_AU	isotropic	sample_conditions_1
jNN-COSY	sample_4_GC	isotropic	sample_conditions_1
2D HCCH-COSY	sample_3_AU	isotropic	sample_conditions_1
2D HCCH-COSY	sample_4_GC	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3_AU	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4_GC	isotropic	sample_conditions_1
2D filtered/edited NOESY	sample_3_AU	isotropic	sample_conditions_1
2D filtered/edited NOESY	sample_4_GC	isotropic	sample_conditions_1
2D-HSQC-TROSY	sample_5_AUGC	isotropic	sample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz
Bruker Avance 800 MHz