BMRB Entry 25772

Name: 1H assignment of Elafin at 288K
Published: 2016-03-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25772

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H assignment of Elafin at 288K

Deposition date:

2015-08-26

Original release date:

2016-03-03

Authors:

Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine

Citation:

Citation: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine. "Complete assignment of 15N, 13C Trappin-2 and 1H assignment of its two domains, Elafin and Cementoin." Biomol. NMR Assign. 10, 223-226 (2016).
PubMed: 26878852

Assembly members:

Assembly members:
Elafin, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Elafin: AQEPVKGPVSTKPGSCPIIL IRCAMLNPPNRCLKDTDCPG IKKCCEGSCGMACFVPQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	388

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Elafin	1

Entities:

Entity 1, Elafin 57 residues - Formula weight is not available

1

ALA

GLN

GLU

PRO

VAL

LYS

GLY

PRO

VAL

SER

2

THR

LYS

PRO

GLY

SER

CYS

PRO

ILE

LEU

3

ILE

ARG

CYS

ALA

MET

LEU

ASN

PRO

ASN

4

ARG

CYS

LEU

LYS

ASP

THR

ASP

CYS

PRO

GLY

5

ILE

LYS

CYS

GLU

GLY

SER

CYS

GLY

6

MET

ALA

CYS

PHE

VAL

PRO

GLN

Samples:

sample_1: Elafin 0.08 mM; sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

Ccpnmr, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz