BMRB Entry 25743

Name: Backbone and side-chain NMR assignments for the Bromodomain of mouse BAZ1A (ACF1)
Published: 2016-10-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25743

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain NMR assignments for the Bromodomain of mouse BAZ1A (ACF1)

Deposition date:

2015-08-07

Original release date:

2016-10-28

Authors:

Fan, Kai

Citation:

Citation: Fan, Kai; Chen, Shengrong; Ge, Yifeng; Ye, Kaiqin; Yao, Qi; Jing, Jun; Zhang, Jiahai; Tu, Xiaoming; Yao, Bing. "Backbone and side-chain NMR assignments for the bromodomain of mouse BAZ1A (ACF1)" Biomol. NMR Assign. 10, 131-134 (2016).
PubMed: 26542424

Assembly members:

Assembly members:
Baz1a-bromodomain, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Baz1a-bromodomain: MNRRSSGRQGGVHELSAFEQ LVVELVRHDDSWPFLKLVSK IQVPDYYDIIKKPIALNIIR EKVNKCEYKLASEFIDDIEL MFSNCFEYNPRNTSEAKAGT RLQAFFHIQAQKLGLHVSPS TVDLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	338
15N chemical shifts	112
1H chemical shifts	715

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Baz1a-bromodomain	1

Entities:

Entity 1, Baz1a-bromodomain 131 residues - Formula weight is not available

1

MET

ASN

ARG

SER

GLY

ARG

GLN

GLY

2

GLY

VAL

HIS

GLU

LEU

SER

ALA

PHE

GLU

GLN

3

LEU

VAL

GLU

LEU

VAL

ARG

HIS

ASP

4

SER

TRP

PRO

PHE

LEU

LYS

LEU

VAL

SER

LYS

5

ILE

GLN

VAL

PRO

ASP

TYR

ASP

ILE

6

LYS

PRO

ILE

ALA

LEU

ASN

ILE

ARG

7

GLU

LYS

VAL

ASN

LYS

CYS

GLU

TYR

LYS

LEU

8

ALA

SER

GLU

PHE

ILE

ASP

ILE

GLU

LEU

9

MET

PHE

SER

ASN

CYS

PHE

GLU

TYR

ASN

PRO

10

ARG

ASN

THR

SER

GLU

ALA

LYS

ALA

GLY

THR

11

ARG

LEU

GLN

ALA

PHE

HIS

ILE

GLN

ALA

12

GLN

LYS

LEU

GLY

LEU

HIS

VAL

SER

PRO

SER

13

THR

VAL

ASP

LEU

GLU

HIS

14

HIS

Samples:

sample_1: Baz1a-bromodomain, [U-99% 13C; U-99% 15N], 0.4 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks