BMRB Entry 25742

Name: TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy
Published: 2016-07-11

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PDB ID: 5aaz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25742
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy

Deposition date:

2015-08-03

Original release date:

2016-07-11

Authors:

Thurston, T.; Allen, Mark; Ravenhill, B.; Karpiyevitch, M.; Bloor, S.; Kaul, A.; Matthews, S.; Komander, D.; Holden, D.; Bycroft, M.; Randow, F.; Matthews, A.

Citation:

Citation: Thurston, Teresa Lm; Boyle, Keith; Allen, Mark; Ravenhill, Benjamin; Karpiyevich, Maryia; Bloor, Stuart; Kaul, Annie; Noad, Jessica; Foeglein, Agnes; Matthews, Sophie; Komander, David; Bycroft, Mark; Randow, Felix. "Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy" EMBO J. 35, 1779-1792 (2016).
PubMed: 27370208

Assembly members:

Assembly members:
OPTINEURIN, polymer, 32 residues, 3514.0982 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: HLTV plasmid

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OPTINEURIN: GSRNIPIHSCPKCGEVLPDI DTLQIHVMDCII

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	104
15N chemical shifts	25
1H chemical shifts	218

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OPTINEURIN	1
2	ZINC ION	2

Entities:

Entity 1, OPTINEURIN 32 residues - 3514.0982 Da.

1

GLY

SER

ARG

ASN

ILE

PRO

ILE

HIS

SER

CYS

2

PRO

LYS

CYS

GLY

GLU

VAL

LEU

PRO

ASP

ILE

3

ASP

THR

LEU

GLN

ILE

HIS

VAL

MET

ASP

CYS

4

ILE

Entity 2, ZINC ION - 65.409 Da.

1

ZN

Samples:

sample_1: OPTINEURIN, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%; Tris 10 mM; NaCl 135 mM; B-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	solution	sample_conditions_1
TOCSY	sample_1	solution	sample_conditions_1
DQF-COSY	sample_1	solution	sample_conditions_1
HSQC	sample_1	solution	sample_conditions_1
HNCACB	sample_1	solution	sample_conditions_1
CBCACONH	sample_1	solution	sample_conditions_1
HNCO	sample_1	solution	sample_conditions_1
HNCACO	sample_1	solution	sample_conditions_1
HNHB	sample_1	solution	sample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

UNP	OPTN_HUMAN
AlphaFold	Q9Y218