BMRB Entry 25712

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PDB ID: 2n5f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25712
MolProbity Validation Chart

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Title:
Solution structure of the dehydroascorbate reductase 3A from Populus trichocarpa
Deposition date:
2015-07-15
Original release date:
2016-03-14
Authors:
Roret, Thomas; Tsan, Pascale
Citation:

Citation: Lallement, Pierre-Alexandre; Roret, Thomas; Tsan, Pascale; M. Gualberto, Jose; Girardet, Jean-Michel; Didierjean, Claude; Rouhier, Nicolas; Hecker, Arnaud. "Biochemical and structural characterization of dehydroascorbate reductases from poplar (Populus trichocarpa), enzymes contributing to the maintenance of the ascorbate pool in plants"  Biochem. J. 473, 717-731 (2016).
PubMed: 26699905

Assembly members:

Assembly members:
Pt-DHAR3A, polymer, 217 residues, 23535.525 Da.

Natural source:

Natural source:   Common Name: black cottonwood   Taxonomy ID: 3694   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus trichocarpa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pt-DHAR3A: MALEICVKAAVGAPNILGDC PFCQRVLLSLEEKKIPYKSH LINLGDKPQWFLEISPEGKV PVVKIDDKWVADSDVIVGIL EEKNPEPPLATPPEFASVGS KIFPSFVKFLKSKDPNDGTE QALLEELKALDGHLKVHGPF IAGEKITAVDLSLAPKLYHL EVALGHFKNWPIPDNLTHVL NYIKLLFSRESFKKTRAAEE HVIAGWEPKVNAHHHHH

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts207
1H chemical shifts1107

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