BMRB Entry 25708
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25708
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Kumar, Atul; Aguirre, Jacob; Condos, Tara; Martinez-Torres, R. Julio; Chaugule, Viduth; Toth, Rachel; Sundaramoorthy, Ramasubramanian; Mercier, Pascal; Knebel, Axel; Spratt, Donald; Barber, Kathryn; Shaw, Gary; Walden, Helen. "Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis" EMBO J. 34, 2506-2521 (2015).
PubMed: 26254304
Assembly members:
Ub_S65E, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a
Entity Sequences (FASTA):
Ub_S65E: SNAMQIFVKTLTGKTITLEV
ESSDTIDNVKSKIQDKEGIP
PDQQRLIFAGKQLEDGRTLS
DYNIQKEETLHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 45 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ub S65E | 1 |
Entities:
Entity 1, Ub S65E 79 residues - Formula weight is not available
| 1 | SER | ASN | ALA | MET | GLN | ILE | PHE | VAL | LYS | THR | ||||
| 2 | LEU | THR | GLY | LYS | THR | ILE | THR | LEU | GLU | VAL | ||||
| 3 | GLU | SER | SER | ASP | THR | ILE | ASP | ASN | VAL | LYS | ||||
| 4 | SER | LYS | ILE | GLN | ASP | LYS | GLU | GLY | ILE | PRO | ||||
| 5 | PRO | ASP | GLN | GLN | ARG | LEU | ILE | PHE | ALA | GLY | ||||
| 6 | LYS | GLN | LEU | GLU | ASP | GLY | ARG | THR | LEU | SER | ||||
| 7 | ASP | TYR | ASN | ILE | GLN | LYS | GLU | GLU | THR | LEU | ||||
| 8 | HIS | LEU | VAL | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ub S65E, [U-99% 13C; U-99% 15N], 300 uM; HEPES 25 mM; Sodium chloride 100 mM; TCEP 500 uM; Imidazole 500 uM; DSS 100 uM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView v8.2.36, Johnson, One Moon Scientific - chemical shift assignment, peak picking
VNMRJ v3.2, Varian - collection
NMRPipe v2010.260.15.01, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz