BMRB Entry 25656

Name: NMR Assignments and structure of Translation initiation factor IF-1 from Burkholderia thailandensis E264.
Published: 2015-06-23

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PDB ID: 2n3s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25656
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignments and structure of Translation initiation factor IF-1 from Burkholderia thailandensis E264.

Deposition date:

2015-06-09

Original release date:

2015-06-23

Authors:

Barnwal, Ravi Pratap; Varani, Gabriele

Citation:

Citation: Barnwal, Ravi Pratap; Varani, Gabriele. "NMR Assignments and structure of Translation initiation factor IF-1 from Burkholderia thailandensis E264." To be Published ., .-..

Assembly members:

Assembly members:
entity, polymer, 72 residues, 8231.756 Da.

Natural source:

Natural source: Common Name: Burkholderia thailandensis Taxonomy ID: 57975 Superkingdom: Bacteria Kingdom: not available Genus/species: Burkholderia thailandensis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-AVA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAKDDVIQMQGEVIENLPNA TFRVKLENGHVVLGHISGKM RMHYIRILPGDKVTVELTPY DLSRARIVFRAK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	281
15N chemical shifts	71
1H chemical shifts	368

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Translation initiation factor IF-1	1

Entities:

Entity 1, Translation initiation factor IF-1 72 residues - 8231.756 Da.

1

MET

ALA

LYS

ASP

VAL

ILE

GLN

MET

GLN

2

GLY

GLU

VAL

ILE

GLU

ASN

LEU

PRO

ASN

ALA

3

THR

PHE

ARG

VAL

LYS

LEU

GLU

ASN

GLY

HIS

4

VAL

LEU

GLY

HIS

ILE

SER

GLY

LYS

MET

5

ARG

MET

HIS

TYR

ILE

ARG

ILE

LEU

PRO

GLY

6

ASP

LYS

VAL

THR

VAL

GLU

LEU

THR

PRO

TYR

7

ASP

LEU

SER

ARG

ALA

ARG

ILE

VAL

PHE

ARG

8

ALA

LYS

Samples:

sample_1: Translation initiation factor IF-1, [U-95% 13C; U-95% 15N], 1.2 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CCPNMR, CCPN - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2N3S
DBJ	BAG44869 BAO87561
EMBL	CAD16707 CAH37203 CAR50566 CBJ36605 CBJ41758
GB	AAU47849 ABA49847 ABB07070 ABC39541 ABD71909
REF	WP_003264140 WP_003273618 WP_004185257 WP_004521905 WP_006577442
SP	A0K3P6 A1V882 A2S7J7 A3MRX5 A3NEF8
AlphaFold	A0K3P6 A1V882 A2S7J7 A3MRX5 A3NEF8