BMRB Entry 25646
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25646
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Citation: Mineev, Konstantin; Goncharuk, Sergey; Kuzmichev, Pavel; Vilar, Marcal; Arseniev, Alexander. "NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs" Biophys. J. 109, 772-782 (2015).
PubMed: 26287629
Assembly members:
p75-TMICD, polymer, 199 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEMEX-1
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 468 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p75-TMICD | 1 |
Entities:
Entity 1, p75-TMICD 199 residues - Formula weight is not available
Sequence numbering corresponds to the Uniprot ID P07174
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | GLY | SER | GLY | ||||
| 2 | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | THR | ARG | ||||
| 3 | GLY | THR | THR | ASP | ASN | LEU | ILE | PRO | VAL | TYR | ||||
| 4 | CYS | SER | ILE | LEU | ALA | ALA | VAL | VAL | VAL | GLY | ||||
| 5 | LEU | VAL | ALA | TYR | ILE | ALA | PHE | LYS | ARG | TRP | ||||
| 6 | ASN | SER | CYS | LYS | GLN | ASN | LYS | GLN | GLY | ALA | ||||
| 7 | ASN | SER | ARG | PRO | VAL | ASN | GLN | THR | PRO | PRO | ||||
| 8 | PRO | GLU | GLY | GLU | LYS | LEU | HIS | SER | ASP | SER | ||||
| 9 | GLY | ILE | SER | VAL | ASP | SER | GLN | SER | LEU | HIS | ||||
| 10 | ASP | GLN | GLN | THR | HIS | THR | GLN | THR | ALA | SER | ||||
| 11 | GLY | GLN | ALA | LEU | LYS | GLY | ASP | GLY | ASN | LEU | ||||
| 12 | TYR | SER | SER | LEU | PRO | LEU | THR | LYS | ARG | GLU | ||||
| 13 | GLU | VAL | GLU | LYS | LEU | LEU | ASN | GLY | ASP | THR | ||||
| 14 | TRP | ARG | HIS | LEU | ALA | GLY | GLU | LEU | GLY | TYR | ||||
| 15 | GLN | PRO | GLU | HIS | ILE | ASP | SER | PHE | THR | HIS | ||||
| 16 | GLU | ALA | CYS | PRO | VAL | ARG | ALA | LEU | LEU | ALA | ||||
| 17 | SER | TRP | GLY | ALA | GLN | ASP | SER | ALA | THR | LEU | ||||
| 18 | ASP | ALA | LEU | LEU | ALA | ALA | LEU | ARG | ARG | ILE | ||||
| 19 | GLN | ARG | ALA | ASP | ILE | VAL | GLU | SER | LEU | CYS | ||||
| 20 | SER | GLU | SER | THR | ALA | THR | SER | PRO | VAL |
Samples:
sample_1: p75-TMICD, [U-99% 13C; U-99% 15N], 0.2 mM; MSP1 0.4 mM; DMPC 19.2 mM; DMPG 4.8 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 70 mM; pH: 6.7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.8, Keller and Wuthrich - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz